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A triphenylamine-based fluorescence probe for detection of hypochlorite in mitochondria.
Yu, Guan-Hua; Hu, Hao-Ran; Liu, Rui-Bin; Sheng, Guo-Zhu; Niu, Jia-Jie; Fang, Ying; Wang, Kun-Peng; Hu, Zhi-Qiang.
Afiliación
  • Yu GH; Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
  • Hu HR; Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
  • Liu RB; Shandong Kangqiao Biotechnology Co. Ltd, Binzhou 256500, China.
  • Sheng GZ; Shandong Kangqiao Biotechnology Co. Ltd, Binzhou 256500, China.
  • Niu JJ; Shandong Kangqiao Biotechnology Co. Ltd, Binzhou 256500, China.
  • Fang Y; Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: fangyingqust@126.com.
  • Wang KP; Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: wangkunpeng@qust.edu.cn.
  • Hu ZQ; Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: huzhiqiang@qust.edu.cn.
Spectrochim Acta A Mol Biomol Spectrosc ; 299: 122830, 2023 Oct 15.
Article en En | MEDLINE | ID: mdl-37178586
The level of HClO/ClO- in mitochondria is essential to keep the normal function of mitochondria. Therefore, it is meaningful to accurately and quickly monitor ClO- in mitochondria. In this work, a new triphenylamine-based fluorescence probe PDTPA was designed and synthesized, in which pyridinium salt and dicyano-vinyl group were introduced as mitochondria targeting site and reaction site for ClO-. The probe showed high sensitivity and fast fluorescence response (<10 s) in the detection of ClO-. Moreover, the probe PDTPA had good linearity in a wide concentration range of ClO- and its detection limit was calculated as 10.5 µM. Confocal fluorescence images demonstrated that the probe could target mitochondria and track the fluctuations of endogenous/exogenous ClO- levels in the mitochondria of living cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Hipocloroso / Colorantes Fluorescentes Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Hipocloroso / Colorantes Fluorescentes Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido