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Electrochemical Lipolysis Induces Adipocyte Death and Fat Necrosis: In Vivo Pilot Study in Pigs.
Pham, Tiffany T; Heidari, Andrew E; Hong, Ellen M; Steward, Earl; Qu, Yueqiao; Chen, Lily Y; Dunn, Brandyn S; Seo, Soo Hong; Syed, Adeela; Dilley, Katelyn; Lee, Lauren; Hutchison, Dana M; Hansen, Kyle D; Patel, Urja; Kim, Sehwan; Hill, Michael G; Wong, Brian J F.
Afiliación
  • Pham TT; From the Beckman Laser Institute & Medical Clinic.
  • Heidari AE; Department of Otolaryngology-Head and Neck Surgery, University of Colorado, School of Medicine.
  • Hong EM; From the Beckman Laser Institute & Medical Clinic.
  • Steward E; Department of Biomedical Engineering.
  • Qu Y; From the Beckman Laser Institute & Medical Clinic.
  • Chen LY; Departments of Surgery.
  • Dunn BS; From the Beckman Laser Institute & Medical Clinic.
  • Seo SH; Department of Biomedical Engineering.
  • Syed A; From the Beckman Laser Institute & Medical Clinic.
  • Dilley K; From the Beckman Laser Institute & Medical Clinic.
  • Lee L; Otolaryngology-Head and Neck Surgery, University of California, Irvine, School of Medicine.
  • Hutchison DM; Department of Dermatology, Korea University, College of Medicine.
  • Hansen KD; Department of Developmental and Cell Biology, University of California, Irvine.
  • Patel U; From the Beckman Laser Institute & Medical Clinic.
  • Kim S; From the Beckman Laser Institute & Medical Clinic.
  • Hill MG; From the Beckman Laser Institute & Medical Clinic.
  • Wong BJF; From the Beckman Laser Institute & Medical Clinic.
Plast Reconstr Surg ; 153(2): 334e-347e, 2024 02 01.
Article en En | MEDLINE | ID: mdl-37163479
BACKGROUND: Current minimally invasive fat reduction modalities use equipment that can cost thousands of U.S. dollars. Electrochemical lipolysis (ECLL), using low-cost battery and electrodes (approximately $10), creates acid/base within fat (width, approximately 3 mm), damaging adipocytes. Longitudinal effects of ECLL have not been studied. In this pilot study, the authors hypothesize that in vivo ECLL induces fat necrosis, decreases adipocyte number/viability, and forms lipid droplets. METHODS: Two female Yorkshire pigs (50 to 60 kg) received ECLL. In pig 1, 10 sites received ECLL, and 10 sites were untreated. In pig 2, 12 sites received ECLL and 12 sites were untreated. For ECLL, two electrodes were inserted into dorsal subcutaneous fat and direct current was applied for 5 minutes. Adverse effects of excessive pain, bleeding, infection, and agitation were monitored. Histology, live-dead (calcein, Hoechst, ethidium homodimer-1), and morphology (Bodipy and Hoechst) assays were performed on day 0 and postprocedure days 1, 2, 7, 14 (pig 1 and pig 2), and 28 (pig 2). Average particle area, fluorescence signal areas, and adipocytes and lipid droplet numbers were compared. RESULTS: No adverse effects occurred. Live-dead assays showed adipocyte death on the anode on days 0 to 7 and the cathode on days 1 to 2 (not significant). Bodipy showed significant adipocyte loss at all sites ( P < 0.001) and lipid droplet formation at the cathode site on day 2 ( P = 0.0046). Histology revealed fat necrosis with significant increases in average particle area at the anode and cathode sites by day 14 (+277.3% change compared with untreated, P < 0.0001; +143.4%, P < 0.0001) and day 28 (+498.6%, P < 0.0001; +354.5%, P < 0.0001). CONCLUSIONS: In vivo ECLL induces fat necrosis in pigs. Further studies are needed to evaluate volumetric fat reduction. CLINICAL RELEVANCE STATEMENT: In vivo ECLL induces adipocyte death and fat necrosis. ECLL has the potential to be utilized in body fat contouring.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Boro / Necrosis Grasa / Lipólisis Límite: Animals Idioma: En Revista: Plast Reconstr Surg Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Boro / Necrosis Grasa / Lipólisis Límite: Animals Idioma: En Revista: Plast Reconstr Surg Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos