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Quantitative chemometric phenotyping of three-dimensional liver organoids by Raman spectral imaging.
LaLone, Vernon; Aizenshtadt, Aleksandra; Goertz, John; Skottvoll, Frøydis Sved; Mota, Marco Barbero; You, Junji; Zhao, Xiaoyu; Berg, Henriette Engen; Stokowiec, Justyna; Yu, Minzhi; Schwendeman, Anna; Scholz, Hanne; Wilson, Steven Ray; Krauss, Stefan; Stevens, Molly M.
Afiliación
  • LaLone V; Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, UK.
  • Aizenshtadt A; Hybrid Technology Hub-Centre of Excellence, Imperial College London, London SW7 2AZ, UK.
  • Goertz J; Hybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, 0317 Oslo, Norway.
  • Skottvoll FS; Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, UK.
  • Mota MB; Hybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, 0317 Oslo, Norway.
  • You J; Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, 0315 Oslo, Norway.
  • Zhao X; Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, UK.
  • Berg HE; Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, UK.
  • Stokowiec J; Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, UK.
  • Yu M; Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, 0315 Oslo, Norway.
  • Schwendeman A; Hybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, 0317 Oslo, Norway.
  • Scholz H; Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI 48109, USA.
  • Wilson SR; Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA.
  • Krauss S; Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI 48109, USA.
  • Stevens MM; Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA.
Cell Rep Methods ; 3(4): 100440, 2023 04 24.
Article en En | MEDLINE | ID: mdl-37159662
Confocal Raman spectral imaging (RSI) enables high-content, label-free visualization of a wide range of molecules in biological specimens without sample preparation. However, reliable quantification of the deconvoluted spectra is needed. Here we develop an integrated bioanalytical methodology, qRamanomics, to qualify RSI as a tissue phantom calibrated tool for quantitative spatial chemotyping of major classes of biomolecules. Next, we apply qRamanomics to fixed 3D liver organoids generated from stem-cell-derived or primary hepatocytes to assess specimen variation and maturity. We then demonstrate the utility of qRamanomics for identifying biomolecular response signatures from a panel of liver-altering drugs, probing drug-induced compositional changes in 3D organoids followed by in situ monitoring of drug metabolism and accumulation. Quantitative chemometric phenotyping constitutes an important step in developing quantitative label-free interrogation of 3D biological specimens.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quimiometría / Hígado Tipo de estudio: Diagnostic_studies Idioma: En Revista: Cell Rep Methods Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quimiometría / Hígado Tipo de estudio: Diagnostic_studies Idioma: En Revista: Cell Rep Methods Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos