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Metagenomic Detection of Divergent Insect- and Bat-Associated Viruses in Plasma from Two African Individuals Enrolled in Blood-Borne Surveillance.
Orf, Gregory S; Olivo, Ana; Harris, Barbara; Weiss, Sonja L; Achari, Asmeeta; Yu, Guixia; Federman, Scot; Mbanya, Dora; James, Linda; Mampunza, Samuel; Chiu, Charles Y; Rodgers, Mary A; Cloherty, Gavin A; Berg, Michael G.
Afiliación
  • Orf GS; Infectious Disease Research, Abbott Diagnostics, Abbott Park, IL 60004, USA.
  • Olivo A; Abbott Pandemic Defense Coalition, Abbott Park, IL 60004, USA.
  • Harris B; Infectious Disease Research, Abbott Diagnostics, Abbott Park, IL 60004, USA.
  • Weiss SL; Abbott Pandemic Defense Coalition, Abbott Park, IL 60004, USA.
  • Achari A; Infectious Disease Research, Abbott Diagnostics, Abbott Park, IL 60004, USA.
  • Yu G; Abbott Pandemic Defense Coalition, Abbott Park, IL 60004, USA.
  • Federman S; Infectious Disease Research, Abbott Diagnostics, Abbott Park, IL 60004, USA.
  • Mbanya D; Abbott Pandemic Defense Coalition, Abbott Park, IL 60004, USA.
  • James L; Abbott Pandemic Defense Coalition, Abbott Park, IL 60004, USA.
  • Mampunza S; Department of Laboratory Medicine, University of California-San Francisco, San Francisco, CA 94143, USA.
  • Chiu CY; Abbott Pandemic Defense Coalition, Abbott Park, IL 60004, USA.
  • Rodgers MA; Department of Laboratory Medicine, University of California-San Francisco, San Francisco, CA 94143, USA.
  • Cloherty GA; Abbott Pandemic Defense Coalition, Abbott Park, IL 60004, USA.
  • Berg MG; Department of Laboratory Medicine, University of California-San Francisco, San Francisco, CA 94143, USA.
Viruses ; 15(4)2023 04 21.
Article en En | MEDLINE | ID: mdl-37113001
Metagenomic next-generation sequencing (mNGS) has enabled the high-throughput multiplexed identification of sequences from microbes of potential medical relevance. This approach has become indispensable for viral pathogen discovery and broad-based surveillance of emerging or re-emerging pathogens. From 2015 to 2019, plasma was collected from 9586 individuals in Cameroon and the Democratic Republic of the Congo enrolled in a combined hepatitis virus and retrovirus surveillance program. A subset (n = 726) of the patient specimens was analyzed by mNGS to identify viral co-infections. While co-infections from known blood-borne viruses were detected, divergent sequences from nine poorly characterized or previously uncharacterized viruses were also identified in two individuals. These were assigned to the following groups by genomic and phylogenetic analyses: densovirus, nodavirus, jingmenvirus, bastrovirus, dicistrovirus, picornavirus, and cyclovirus. Although of unclear pathogenicity, these viruses were found circulating at high enough concentrations in plasma for genomes to be assembled and were most closely related to those previously associated with bird or bat excrement. Phylogenetic analyses and in silico host predictions suggested that these are invertebrate viruses likely transmitted through feces containing consumed insects or through contaminated shellfish. This study highlights the power of metagenomics and in silico host prediction in characterizing novel viral infections in susceptible individuals, including those who are immunocompromised from hepatitis viruses and retroviruses, or potentially exposed to zoonotic viruses from animal reservoir species.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus / Virosis / Quirópteros / Coinfección Tipo de estudio: Diagnostic_studies / Risk_factors_studies / Screening_studies Límite: Animals Idioma: En Revista: Viruses Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus / Virosis / Quirópteros / Coinfección Tipo de estudio: Diagnostic_studies / Risk_factors_studies / Screening_studies Límite: Animals Idioma: En Revista: Viruses Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza