Your browser doesn't support javascript.
loading
Bioactive Metabolite from Endophytic Aspergillus versicolor SB5 with Anti-Acetylcholinesterase, Anti-Inflammatory and Antioxidant Activities: In Vitro and In Silico Studies.
Elawady, Mohamed E; Hamed, Ahmed A; Alsallami, Wamedh M; Gabr, Ebtsam Z; Abdel-Monem, Mohamed O; Hassan, Mervat G.
Afiliación
  • Elawady ME; Microbial Biotechnology Department, National Research Centre, El-Buhouth St. 33, Cairo 12622, Egypt.
  • Hamed AA; Microbial Chemistry Department, National Research Centre, El-Buhouth St. 33, Cairo 12622, Egypt.
  • Alsallami WM; Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt.
  • Gabr EZ; Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt.
  • Abdel-Monem MO; Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt.
  • Hassan MG; Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt.
Microorganisms ; 11(4)2023 Apr 19.
Article en En | MEDLINE | ID: mdl-37110485
Endophytic fungi are a highly unpredictable group of microorganisms that can create a diverse range of secondary metabolites with biological activity. These metabolites enhance the host's ability to tolerate stress caused by various factors, such as disease, insects, pathogens, and herbivores. The secondary metabolites produced by endophytic fungi may have potential applications in agriculture, pharmacy, and medicine. The purpose of this study was to examine the anti-acetylcholinesterase activity of secondary metabolites extracted from endophytic fungi. Aspergillus versicolor SB5 was one of the many endophytic fungi isolated from Juncus rigidus and identified genetically with accession number ON872302. Our study utilized fermentation and microbial cultivation techniques to obtain secondary metabolites. During the course of our investigation, we isolated a compound called Physcion (C1) from the endophytic fungus Aspergillus versicolor SB5. We subsequently identified that C1 possesses inhibitory activity against COX-2 and LOX-1, with IC50 values of 43.10 and 17.54 µg/mL, respectively, making it an effective anti-inflammatory agent. Moreover, we found that C1 also exhibited potent anticholinesterase activity (86.9 ± 1.21%). In addition to these promising therapeutic properties, our experiments demonstrated that C1 possesses strong antioxidant capacity, as evidenced by its ability to scavenge DPPH, ABTS, O2 radicals, and NO and inhibit lipid peroxidation. To further investigate the molecular mechanisms underlying C1 pharmacological properties, we employed SwissADME web tools to predict the compound's ADME-related physicochemical properties and used Molecular Operating Environment and PyMOL for molecular docking studies.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Microorganisms Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Microorganisms Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza