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Diagnostic performance of RASSF1A and SHOX2 methylation combined with EGFR mutations for differentiation between small pulmonary nodules.
Ji, Xiang-Yu; Li, Hong; Chen, Hui-Hui; Lin, Jie.
Afiliación
  • Ji XY; Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
  • Li H; Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, People's Republic of China.
  • Chen HH; Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
  • Lin J; Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
J Cancer Res Clin Oncol ; 149(11): 8557-8571, 2023 Sep.
Article en En | MEDLINE | ID: mdl-37097393
BACKGROUND AND AIM: Aberrant methylation of Ras association domain family 1, isoform A (RASSF1A), and short-stature homeobox gene 2 (SHOX2) promoters has been validated as a pair of valuable biomarkers for diagnosing early lung adenocarcinomas (LUADs). Epidermal growth factor receptor (EGFR) is the key driver mutation in lung carcinogenesis. This study aimed to investigate the aberrant promoter methylation of RASSF1A and SHOX2, and the genetic mutation of EGFR in 258 specimens of early LUADs. METHODS: We retrospectively selected 258 paraffin-embedded samples of pulmonary nodules measuring 2 cm or less in diameter and evaluated the diagnostic performance of individual biomarker assays and multiple panels between noninvasive (group 1) and invasive lesions (groups 2A and 2B). Then, we investigated the interaction between genetic and epigenetic alterations. RESULTS: The degree of RASSF1A and SHOX2 promoter methylation and EGFR mutation was significantly higher in invasive lesions than in noninvasive lesions. The three biomarkers distinguished between noninvasive and invasive lesions with reliable sensitivity and specificity: 60.9% sensitivity [95% confidence interval (CI) 52.41-68.78] and 80.0% specificity (95% CI 72.14-86.07). The novel panel biomarkers could further discriminate among three invasive pathological subtypes (area under the curve value > 0.6). The distribution of RASSF1A methylation and EGFR mutation was considerably exclusive in early LUAD (P = 0.002). CONCLUSION: DNA methylation of RASSF1A and SHOX2 is a pair of promising biomarkers, which may be used in combination with other driver alterations, such as EGFR mutation, to support the differential diagnosis of LUADs, especially for stage I.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nódulos Pulmonares Múltiples / Adenocarcinoma del Pulmón / Neoplasias Pulmonares Tipo de estudio: Diagnostic_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: J Cancer Res Clin Oncol Año: 2023 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nódulos Pulmonares Múltiples / Adenocarcinoma del Pulmón / Neoplasias Pulmonares Tipo de estudio: Diagnostic_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: J Cancer Res Clin Oncol Año: 2023 Tipo del documento: Article Pais de publicación: Alemania