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First draft genome and transcriptome of Cercosporidium personatum, causal agent of late leaf spot disease of peanut.
Arias, Renee S; Dobbs, John T; Stewart, Jane E; Cantonwine, Emily G; Orner, Valerie A; Sobolev, Victor S; Lamb, Marshall C; Massa, Alicia N.
Afiliación
  • Arias RS; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Peanut Research Laboratory (NPRL), 1011 Forrester dr. S.E., Dawson, GA, USA. renee.arias@usda.gov.
  • Dobbs JT; Department of Agricultural Biology, Colorado State University, Fort Collins, CO, USA.
  • Stewart JE; Department of Agricultural Biology, Colorado State University, Fort Collins, CO, USA.
  • Cantonwine EG; Department of Biology, Valdosta State University, Valdosta, GA, USA.
  • Orner VA; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Peanut Research Laboratory (NPRL), 1011 Forrester dr. S.E., Dawson, GA, USA.
  • Sobolev VS; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Peanut Research Laboratory (NPRL), 1011 Forrester dr. S.E., Dawson, GA, USA.
  • Lamb MC; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Peanut Research Laboratory (NPRL), 1011 Forrester dr. S.E., Dawson, GA, USA.
  • Massa AN; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Peanut Research Laboratory (NPRL), 1011 Forrester dr. S.E., Dawson, GA, USA.
BMC Res Notes ; 16(1): 58, 2023 Apr 21.
Article en En | MEDLINE | ID: mdl-37085927
OBJECTIVE: Two main fungal leaf spot diseases occur in peanut, namely early leaf spot (ELS) and late leaf spot (LLS), these cause a yearly average of $44 million losses. Limited genetic information, 3534 bp of sequencing, exists about the causal agent of LLS, Cercosporidium personatum (syn. Nothopassalora personata, syn. Phaeoisariopsis personata). The extremely slow growth of this fungus, approximately 1 cm colony in 6 months, and challenges in nucleic acid extractions have hindered research on LLS. Our goal in this work is to provide a reference genome for research on this pathogen. RESULTS: Whole genome and transcriptome sequencing of the LLS fungus were obtained. A total of 233,542,110 reads of the genome were de novo assembled resulting in 1061 scaffolds, and estimated genome size 27,597,787 bp. RNA sequencing resulted in 11,848,198 reads that were de novo assembled into 13,343 contigs. Genome annotation resulted in 10,703 putative genes. BUSCO analysis of the genome and annotation resulted in 91.1% and 89.5% completeness, respectively. Phylogenetic dendrograms for 5442 bp and 4401 bp of RNA Polymerase II largest and second largest subunits, and for 5474 bp of the ribosomal RNA cistron of C. personatum are presented in relation to closely related fungi.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ascomicetos / Fabaceae Idioma: En Revista: BMC Res Notes Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ascomicetos / Fabaceae Idioma: En Revista: BMC Res Notes Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido