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Cell wall and cytoskeletal contributions in single cell biomechanics of Nicotiana tabacum.
Ginsberg, Leah; McDonald, Robin; Lin, Qinchen; Hendrickx, Rodinde; Spigolon, Giada; Ravichandran, Guruswami; Daraio, Chiara; Roumeli, Eleftheria.
Afiliación
  • Ginsberg L; Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USA.
  • McDonald R; Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USA.
  • Lin Q; Department of Materials Science and Engineering, University of Washington, Seattle, WA 98195, USA.
  • Hendrickx R; Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USA.
  • Spigolon G; Biological Imaging Facility, California Institute of Technology, Pasadena, CA 91125, USA.
  • Ravichandran G; Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USA.
  • Daraio C; Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USA.
  • Roumeli E; Department of Materials Science and Engineering, University of Washington, Seattle, WA 98195, USA.
Quant Plant Biol ; 3: e1, 2022.
Article en En | MEDLINE | ID: mdl-37077972
Studies on the mechanics of plant cells usually focus on understanding the effects of turgor pressure and properties of the cell wall (CW). While the functional roles of the underlying cytoskeleton have been studied, the extent to which it contributes to the mechanical properties of cells is not elucidated. Here, we study the contributions of the CW, microtubules (MTs) and actin filaments (AFs), in the mechanical properties of Nicotiana tabacum cells. We use a multiscale biomechanical assay comprised of atomic force microscopy and micro-indentation in solutions that (i) remove MTs and AFs and (ii) alter osmotic pressures in the cells. To compare measurements obtained by the two mechanical tests, we develop two generative statistical models to describe the cell's behaviour using one or both datasets. Our results illustrate that MTs and AFs contribute significantly to cell stiffness and dissipated energy, while confirming the dominant role of turgor pressure.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: Quant Plant Biol Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: Quant Plant Biol Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido