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Epidemiology of Enteroaggregative, Enteropathogenic, and Shiga Toxin-Producing Escherichia coli Among Children Aged <5 Years in 3 Countries in Africa, 2015-2018: Vaccine Impact on Diarrhea in Africa (VIDA) Study.
Ochieng, John B; Powell, Helen; Sugerman, Ciara E; Omore, Richard; Ogwel, Billy; Juma, Jane; Awuor, Alex O; Sow, Samba O; Sanogo, Doh; Onwuchekwa, Uma; Keita, Adama Mamby; Traoré, Awa; Badji, Henry; Hossain, M Jahangir; Jones, Joquina Chiquita M; Kasumba, Irene N; Nasrin, Dilruba; Roose, Anna; Liang, Yuanyuan; Jamka, Leslie P; Antonio, Martin; Platts-Mills, James A; Liu, Jie; Houpt, Eric R; Mintz, Eric D; Hunsperger, Elizabeth; Onyango, Clayton O; Strockbine, Nancy; Widdowson, Marc-Alain; Verani, Jennifer R; Tennant, Sharon M; Kotloff, Karen L.
Afiliación
  • Ochieng JB; Kenya Medical Research Institute, Center for Global Health Research, Kisumu, Kenya.
  • Powell H; Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Sugerman CE; Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Omore R; Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Ogwel B; Kenya Medical Research Institute, Center for Global Health Research, Kisumu, Kenya.
  • Juma J; Kenya Medical Research Institute, Center for Global Health Research, Kisumu, Kenya.
  • Awuor AO; Kenya Medical Research Institute, Center for Global Health Research, Kisumu, Kenya.
  • Sow SO; Kenya Medical Research Institute, Center for Global Health Research, Kisumu, Kenya.
  • Sanogo D; Centre pour le Développement des Vaccins du Mali, Bamako, Mali.
  • Onwuchekwa U; Centre pour le Développement des Vaccins du Mali, Bamako, Mali.
  • Keita AM; Centre pour le Développement des Vaccins du Mali, Bamako, Mali.
  • Traoré A; Centre pour le Développement des Vaccins du Mali, Bamako, Mali.
  • Badji H; Centre pour le Développement des Vaccins du Mali, Bamako, Mali.
  • Hossain MJ; Medical Research Council Unit, The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The Gambia.
  • Jones JCM; Medical Research Council Unit, The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The Gambia.
  • Kasumba IN; Medical Research Council Unit, The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The Gambia.
  • Nasrin D; Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Roose A; Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Liang Y; Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Jamka LP; Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Antonio M; Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Platts-Mills JA; Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Liu J; Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Houpt ER; Department of Epidemiology and Public Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Mintz ED; Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Hunsperger E; Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Onyango CO; Medical Research Council Unit, The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The Gambia.
  • Strockbine N; Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA.
  • Widdowson MA; Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA.
  • Verani JR; Department of Microbial Surveillance and Biosafety, School of Public Health, Qingdao University, Qingdao, China.
  • Tennant SM; Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA.
  • Kotloff KL; Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Clin Infect Dis ; 76(76 Suppl1): S77-S86, 2023 04 19.
Article en En | MEDLINE | ID: mdl-37074433
BACKGROUND: To address knowledge gaps regarding diarrheagenic Escherichia coli (DEC) in Africa, we assessed the clinical and epidemiological features of enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), and Shiga toxin-producing E. coli (STEC) positive children with moderate-to-severe diarrhea (MSD) in Mali, The Gambia, and Kenya. METHODS: Between May 2015 and July 2018, children aged 0-59 months with medically attended MSD and matched controls without diarrhea were enrolled. Stools were tested conventionally using culture and multiplex polymerase chain reaction (PCR), and by quantitative PCR (qPCR). We assessed DEC detection by site, age, clinical characteristics, and enteric coinfection. RESULTS: Among 4840 children with MSD and 6213 matched controls enrolled, 4836 cases and 1 control per case were tested using qPCR. Of the DEC detected with TAC, 61.1% were EAEC, 25.3% atypical EPEC (aEPEC), 22.4% typical EPEC (tEPEC), and 7.2% STEC. Detection was higher in controls than in MSD cases for EAEC (63.9% vs 58.3%, P < .01), aEPEC (27.3% vs 23.3%, P < .01), and STEC (9.3% vs 5.1%, P < .01). EAEC and tEPEC were more frequent in children aged <23 months, aEPEC was similar across age strata, and STEC increased with age. No association between nutritional status at follow-up and DEC pathotypes was found. DEC coinfection with Shigella/enteroinvasive E. coli was more common among cases (P < .01). CONCLUSIONS: No significant association was detected between EAEC, tEPEC, aEPEC, or STEC and MSD using either conventional assay or TAC. Genomic analysis may provide a better definition of the virulence factors associated with diarrheal disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Escherichia coli / Escherichia coli Enteropatógena / Escherichia coli Shiga-Toxigénica / Coinfección Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Child / Humans País/Región como asunto: Africa Idioma: En Revista: Clin Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2023 Tipo del documento: Article País de afiliación: Kenia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Escherichia coli / Escherichia coli Enteropatógena / Escherichia coli Shiga-Toxigénica / Coinfección Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Child / Humans País/Región como asunto: Africa Idioma: En Revista: Clin Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2023 Tipo del documento: Article País de afiliación: Kenia Pais de publicación: Estados Unidos