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Kinetic and mechanistic diversity of intestinal immune homeostasis characterized by rapid removal of gut bacteria.
Jung, Da-Jung; Gebremedhin, Ledia; Goh, Byoungsook; Yoo, Ji-Sun; Gazzaniga, Francesca; Kasper, Dennis L; Oh, Sungwhan F.
Afiliación
  • Jung DJ; Center for Experimental Therapeutics and reperfusion injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Boston, MA, USA.
  • Gebremedhin L; Center for Experimental Therapeutics and reperfusion injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Boston, MA, USA.
  • Goh B; Center for Experimental Therapeutics and reperfusion injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Boston, MA, USA.
  • Yoo JS; Center for Experimental Therapeutics and reperfusion injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Boston, MA, USA.
  • Gazzaniga F; Department of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
  • Kasper DL; Department of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
  • Oh SF; Center for Experimental Therapeutics and reperfusion injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Boston, MA, USA.
Gut Microbes ; 15(1): 2201154, 2023.
Article en En | MEDLINE | ID: mdl-37069732
Symbiotic microbiota critically contribute to host immune homeostasis in effector cell-specific manner. For exclusion of microbial component, germ-free animals have been the gold standard method. However, total removal of the entire gut microbiota of an animal from birth significantly skews physiological development. On the other hand, removal of gut microbiota from conventional mice using oral antibiotics has its own limitations, especially lack of consistency and the requirement for long-term treatment period. Here, we introduce an improved regimen to quickly remove gut microbiota and to maintain sterility, that is well received by animals without refusal. Rapid and consistent exclusion of resident bacteria in the gut lumen revealed kinetic differences among colonic lymphocyte subsets, which cannot be observed with typical germ-free animal models. Furthermore, the proposed method distinguished the mechanism of microbiota contribution as a direct stimulus to capable effector cells and a homeostatic cue to maintain such cell types.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiota / Microbioma Gastrointestinal Límite: Animals Idioma: En Revista: Gut Microbes Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiota / Microbioma Gastrointestinal Límite: Animals Idioma: En Revista: Gut Microbes Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos