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C-type lectin receptor agonists elicit functional IL21-expressing Tfh cells and induce primary B cell responses in neonates.
Vono, Maria; Mastelic-Gavillet, Beatris; Mohr, Elodie; Östensson, Malin; Persson, Josefine; Olafsdottir, Thorunn A; Lemeille, Sylvain; Pejoski, David; Hartley, Oliver; Christensen, Dennis; Andersen, Peter; Didierlaurent, Arnaud M; Harandi, Ali M; Lambert, Paul-Henri; Siegrist, Claire-Anne.
Afiliación
  • Vono M; Center for Vaccine Immunology, Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland.
  • Mastelic-Gavillet B; Center for Vaccine Immunology, Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland.
  • Mohr E; Center for Vaccine Immunology, Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland.
  • Östensson M; Department of Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden.
  • Persson J; Department of Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden.
  • Olafsdottir TA; Faculty of Medicine, Biomedical Center, University of Iceland, Reykjavik, Iceland.
  • Lemeille S; Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland.
  • Pejoski D; Center for Vaccine Immunology, Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland.
  • Hartley O; Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland.
  • Christensen D; Vaccine Adjuvant Research, Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark.
  • Andersen P; Vaccine Adjuvant Research, Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark.
  • Didierlaurent AM; Center for Vaccine Immunology, Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland.
  • Harandi AM; Department of Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden.
  • Lambert PH; Vaccine Evaluation Center, British Columbia (BC) Children's Hospital Research Institute, University of British Columbia, Vancouver, BC, Canada.
  • Siegrist CA; Center for Vaccine Immunology, Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland.
Front Immunol ; 14: 1155200, 2023.
Article en En | MEDLINE | ID: mdl-37063899
Introduction: C-type lectin receptor (CLR) agonists emerged as superior inducers of primary B cell responses in early life compared with Toll-like receptor (TLR) agonists, while both types of adjuvants are potent in adults. Methods: Here, we explored the mechanisms accounting for the differences in neonatal adjuvanticity between a CLR-based (CAF®01) and a TLR4-based (GLA-SE) adjuvant administered with influenza hemagglutinin (HA) in neonatal mice, by using transcriptomics and systems biology analyses. Results: On day 7 after immunization, HA/CAF01 increased IL6 and IL21 levels in the draining lymph nodes, while HA/GLA-SE increased IL10. CAF01 induced mixed Th1/Th17 neonatal responses while T cell responses induced by GLA-SE had a more pronounced Th2-profile. Only CAF01 induced T follicular helper (Tfh) cells expressing high levels of IL21 similar to levels induced in adult mice, which is essential for germinal center (GC) formation. Accordingly, only CAF01- induced neonatal Tfh cells activated adoptively transferred hen egg lysozyme (HEL)-specific B cells to form HEL+ GC B cells in neonatal mice upon vaccination with HEL-OVA. Discussion: Collectively, the data show that CLR-based adjuvants are promising neonatal and infant adjuvants due to their ability to harness Tfh responses in early life.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Centro Germinal / Lectinas Tipo C / Células T Auxiliares Foliculares Límite: Animals Idioma: En Revista: Front Immunol Año: 2023 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Centro Germinal / Lectinas Tipo C / Células T Auxiliares Foliculares Límite: Animals Idioma: En Revista: Front Immunol Año: 2023 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Suiza