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Analysis of RNA Polyadenylation in Healthy and Osteoarthritic Human Articular Cartilage.
Winstanley-Zarach, Phaedra; Rot, Gregor; Kuba, Shweta; Smagul, Aibek; Peffers, Mandy J; Tew, Simon R.
Afiliación
  • Winstanley-Zarach P; Centre for Integrated Research into Musculoskeletal Ageing (CIMA), Department of Musculoskeletal and Ageing Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool L7 8TX, UK.
  • Rot G; Institute of Molecular Life Sciences, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
  • Kuba S; Swiss Institute of Bioinformatics, Amphipôle, Quartier UNIL-Sorge, 1015 Lausanne, Switzerland.
  • Smagul A; Centre for Integrated Research into Musculoskeletal Ageing (CIMA), Department of Musculoskeletal and Ageing Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool L7 8TX, UK.
  • Peffers MJ; School of Health and Life Sciences, National Horizons Centre, Teesside University, Darlington DL1 1HG, UK.
  • Tew SR; Centre for Integrated Research into Musculoskeletal Ageing (CIMA), Department of Musculoskeletal and Ageing Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool L7 8TX, UK.
Int J Mol Sci ; 24(7)2023 Apr 01.
Article en En | MEDLINE | ID: mdl-37047586
Polyadenylation (polyA) defines the 3' boundary of a transcript's genetic information. Its position can vary and alternative polyadenylation (APA) transcripts can exist for a gene. This causes variance in 3' regulatory domains and can affect coding sequence if intronic events occur. The distribution of polyA sites on articular chondrocyte transcripts has not been studied so we aimed to define their transcriptome-wide location in age-matched healthy and osteoarthritic knee articular cartilage. Total RNA was isolated from frozen tissue samples and analysed using the QuantSeq-Reverse 3' RNA sequencing approach, where each read runs 3' to 5' from within the polyA tail into the transcript and contains a distinct polyA site. Differential expression of transcripts was significant altered between healthy and osteoarthritic samples with enrichment for functionalities that were strongly associated with joint pathology. Subsequent examination of polyA site data allowed us to define the extent of site usage across all the samples. When comparing healthy and osteoarthritic samples, we found that differential use of polyadenylation sites was modest. However, in the genes affected, there was potential for the APA to have functional relevance. We have characterised the polyadenylation landscape of human knee articular chondrocytes and conclude that osteoarthritis does not elicit a widespread change in their polyadenylation site usage. This finding differentiates knee osteoarthritis from pathologies such as cancer where APA is more commonly observed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cartílago Articular / Osteoartritis de la Rodilla Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cartílago Articular / Osteoartritis de la Rodilla Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article Pais de publicación: Suiza