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Tissue-like environments shape functional interactions of HIV-1 with immature dendritic cells.
Gallucci, Lara; Abele, Tobias; Fronza, Raffaele; Stolp, Bettina; Laketa, Vibor; Sid Ahmed, Samy; Flemming, Annica; Müller, Barbara; Göpfrich, Kerstin; Fackler, Oliver T.
Afiliación
  • Gallucci L; Department of Infectious Diseases, Integrative Virology, CIID, University Hospital Heidelberg, Heidelberg, Germany.
  • Abele T; Biophysical Engineering Group, Max Planck Institute for Medical Research, Heidelberg, Germany.
  • Fronza R; ZMBH, Center for Molecular Biology, Heidelberg University, Heidelberg, Germany.
  • Stolp B; ProtaGene CGT GmbH, Heidelberg, Germany.
  • Laketa V; Department of Infectious Diseases, Integrative Virology, CIID, University Hospital Heidelberg, Heidelberg, Germany.
  • Sid Ahmed S; Department of Infectious Diseases, Virology, CIID, Heidelberg University Hospital, Heidelberg, Germany.
  • Flemming A; German Centre for Infection Research (DZIF), Partner Site Heidelberg, Heidelberg, Germany.
  • Müller B; Department of Infectious Diseases, Integrative Virology, CIID, University Hospital Heidelberg, Heidelberg, Germany.
  • Göpfrich K; Department of Infectious Diseases, Virology, CIID, Heidelberg University Hospital, Heidelberg, Germany.
  • Fackler OT; Department of Infectious Diseases, Virology, CIID, Heidelberg University Hospital, Heidelberg, Germany.
EMBO Rep ; 24(6): e56818, 2023 06 05.
Article en En | MEDLINE | ID: mdl-37042686
Immature dendritic cells (iDCs) migrate in microenvironments with distinct cell and extracellular matrix densities in vivo and contribute to HIV-1 dissemination and mounting of antiviral immune responses. Here, we find that, compared to standard 2D suspension cultures, 3D collagen as tissue-like environment alters iDC properties and their response to HIV-1 infection. iDCs adopt an elongated morphology with increased deformability in 3D collagen at unaltered activation, differentiation, cytokine secretion, or responsiveness to LPS. While 3D collagen reduces HIV-1 particle uptake by iDCs, fusion efficiency is increased to elevate productive infection rates due to elevated cell surface exposure of the HIV-1-binding receptor DC-SIGN. In contrast, 3D collagen reduces HIV transfer to CD4 T cells from iDCs. iDC adaptations to 3D collagen include increased pro-inflammatory cytokine production and reduced antiviral gene expression in response to HIV-1 infection. Adhesion to a 2D collagen matrix is sufficient to increase iDC deformability, DC-SIGN exposure, and permissivity to HIV-1 infection. Thus, mechano-physical cues of 2D and 3D tissue-like collagen environments regulate iDC function and shape divergent roles during HIV-1 infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por VIH / VIH-1 Límite: Humans Idioma: En Revista: EMBO Rep Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por VIH / VIH-1 Límite: Humans Idioma: En Revista: EMBO Rep Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido