Complexities and benefits of adopting next-generation sequencing-based tuberculosis diagnostics: a qualitative study among stakeholders in low and high-income countries.

Grandjean Lapierre, Simon; Nouvet, Elysée; Boutin, Catherine-Audrey; Rabodoarivelo, Marie-Sylvianne; Mahboob, Omar; Smith, Maxwell J; Rakotosamimanana, Niaina

Grandjean Lapierre, Simon; Nouvet, Elysée; Boutin, Catherine-Audrey; Rabodoarivelo, Marie-Sylvianne; Mahboob, Omar; Smith, Maxwell J; Rakotosamimanana, Niaina.

Afiliación

Grandjean Lapierre S; Mycobacteria Unit, Institut Pasteur Madagascar, Antananarivo, Madagascar.
Nouvet E; Microbiology, Infectious Diseases and Immunology Department, Université de Montréal, Montreal, Québec, Canada.
Boutin CA; School of Health Studies, Western University, London, Ontario, Canada enouvet@uwo.ca.
Rabodoarivelo MS; Microbiology, Infectious Diseases and Immunology Department, Université de Montréal, Montreal, Québec, Canada.
Mahboob O; Mycobacteria Unit, Institut Pasteur Madagascar, Antananarivo, Madagascar.
Smith MJ; Departamento de Microbiología, Pediatría, Radiología y Salud Pública, Universidad de Zaragoza, Zaragoza, Spain.
Rakotosamimanana N; Florida State University College of Medicine, Florida State University, Tallahassee, Florida, USA.

BMJ Open ; 13(4): e066651, 2023 04 10.

Article en En | MEDLINE | ID: mdl-37037623

RESUMEN

OBJECTIVES: To clarify perceived benefits, barriers and facilitators of Mycobacterium tuberculosis next-generation sequencing implementation in Madagascar and Canada, towards informing implementation of this diagnostic technology in public health agencies and clinical settings in and beyond these settings. DESIGN: This qualitative study involved conducting semistructured interviews with key stakeholders engaged with next-generation sequencing implementation in Madagascar and Canada. Team-based descriptive analysis supported by Nvivo V.12.0 was used to identify key themes. SETTING: The study was conducted with participants involved at the clinical, diagnostic and surveillance levels of tuberculosis (TB) management from Madagascar and Canada. PARTICIPANTS: Eighteen participants were interviewed (nine Madagascar and nine Canada) and included individuals purposively sampled based on involvement with TB surveillance, laboratory diagnosis and clinical management. RESULTS: The following five themes emerged in the analysis of Malagasy and Canadian interviews: (1) heterogeneity in experience with established TB diagnostics, (2) variable understanding of new sequencing-based diagnostics potential; (3) further evidence as being key to expand adoption; (4) ethical arguments and concerns; (5) operational and system-level considerations. CONCLUSION: There persists important lack of familiarity with TB next-generation sequencing (TB NGS) applications among stakeholders in Canada and Madagascar. This translates into skepticism on the evidence underlying its use and its true potential value added within global public health systems. If deployed, TB NGS testing should be integrated with clinical and surveillance programmes. Although this is perceived as a priority, leadership and funding responsibilities for this integration to happen remains unclear to clinical, laboratory and public health stakeholders.

Asunto(s)

Tuberculosis; Humanos; Países Desarrollados; Canadá; Tuberculosis/diagnóstico; Investigación Cualitativa; Secuenciación de Nucleótidos de Alto Rendimiento

Palabras clave

diagnostic microbiology; qualitative research; tuberculosis

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis Tipo de estudio: Diagnostic_studies / Prognostic_studies / Qualitative_research Aspecto: Ethics Límite: Humans País/Región como asunto: America do norte Idioma: En Revista: BMJ Open Año: 2023 Tipo del documento: Article País de afiliación: Madagascar Pais de publicación: Reino Unido

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