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AccsHSP21.7 enhances the antioxidant capacity of Apis cerana cerana.
Huang, Yuanyuan; Zhang, Yuanying; Niu, Xiaojing; Sun, Yunhao; Wang, Hongfang; Guo, Xingqi; Xu, Baohua; Wang, Chen.
Afiliación
  • Huang Y; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China.
  • Zhang Y; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China.
  • Niu X; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China.
  • Sun Y; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China.
  • Wang H; College of Animal Science and Technology, Shandong Agricultural University, Taian, China.
  • Guo X; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China.
  • Xu B; College of Animal Science and Technology, Shandong Agricultural University, Taian, China.
  • Wang C; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China.
J Sci Food Agric ; 103(11): 5401-5411, 2023 Aug 30.
Article en En | MEDLINE | ID: mdl-37029991
BACKGROUND: The widespread use of glyphosate has many adverse effects on Apis cerana cerana. Due to the incomplete understanding of the molecular mechanisms of glyphosate toxicity, there are no available methods for mitigating the threat of glyphosate to Apis cerana cerana. Small heat shock proteins (sHSPs) play an important role in resisting oxidative stress, but their mechanism of action in Apis cerana cerana remains unclear. RESULTS: In this experiment, we cloned and identified AccsHSP21.7. Studies have shown that AccsHSP21.7 contains binding motifs for various transcription factors related to oxidative stress. Abiotic stresses induced the expression of AccsHSP21.7. Bacteriostatic testing of a recombinant AccsHSP21.7 protein proved that Escherichia coli overexpressing AccsHSP21.7 showed increased resistance to oxidative stress. Knocking down the AccsHSP21.7 gene caused significant damage to midgut cells, which seriously disrupted the antioxidant system in Apis cerana cerana and greatly increased mortality under glyphosate stress. CONCLUSION: This study investigated the relationship between antioxidant regulation and the AccsHSP21.7 gene at the molecular level, and the results have guiding significance for the improvement of stress resistance in Apis cerana cerana. © 2023 Society of Chemical Industry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Oxidativo / Antioxidantes Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Sci Food Agric Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Oxidativo / Antioxidantes Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Sci Food Agric Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido