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Acute manipulation and real-time visualization of membrane trafficking and exocytosis in Drosophila.
Glashauser, Jade; Camelo, Carolina; Hollmann, Manuel; Backer, Wilko; Jacobs, Thea; Sanchez, Jone Isasti; Schleutker, Raphael; Förster, Dominique; Berns, Nicola; Riechmann, Veit; Luschnig, Stefan.
Afiliación
  • Glashauser J; Institute of Integrative Cell Biology and Physiology, Faculty of Biology and Cells in Motion (CiM) Interfaculty Center, University of Münster, 48149 Münster, Germany.
  • Camelo C; Institute of Integrative Cell Biology and Physiology, Faculty of Biology and Cells in Motion (CiM) Interfaculty Center, University of Münster, 48149 Münster, Germany.
  • Hollmann M; Institute of Integrative Cell Biology and Physiology, Faculty of Biology and Cells in Motion (CiM) Interfaculty Center, University of Münster, 48149 Münster, Germany.
  • Backer W; Institute of Integrative Cell Biology and Physiology, Faculty of Biology and Cells in Motion (CiM) Interfaculty Center, University of Münster, 48149 Münster, Germany.
  • Jacobs T; Institute of Integrative Cell Biology and Physiology, Faculty of Biology and Cells in Motion (CiM) Interfaculty Center, University of Münster, 48149 Münster, Germany.
  • Sanchez JI; Institute of Integrative Cell Biology and Physiology, Faculty of Biology and Cells in Motion (CiM) Interfaculty Center, University of Münster, 48149 Münster, Germany.
  • Schleutker R; Institute of Integrative Cell Biology and Physiology, Faculty of Biology and Cells in Motion (CiM) Interfaculty Center, University of Münster, 48149 Münster, Germany.
  • Förster D; Institute of Integrative Cell Biology and Physiology, Faculty of Biology and Cells in Motion (CiM) Interfaculty Center, University of Münster, 48149 Münster, Germany.
  • Berns N; Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
  • Riechmann V; Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
  • Luschnig S; Institute of Integrative Cell Biology and Physiology, Faculty of Biology and Cells in Motion (CiM) Interfaculty Center, University of Münster, 48149 Münster, Germany. Electronic address: luschnig@uni-muenster.de.
Dev Cell ; 58(8): 709-723.e7, 2023 04 24.
Article en En | MEDLINE | ID: mdl-37023749
Intracellular trafficking of secretory proteins plays key roles in animal development and physiology, but so far, tools for investigating the dynamics of membrane trafficking have been limited to cultured cells. Here, we present a system that enables acute manipulation and real-time visualization of membrane trafficking through the reversible retention of proteins in the endoplasmic reticulum (ER) in living multicellular organisms. By adapting the "retention using selective hooks" (RUSH) approach to Drosophila, we show that trafficking of GPI-linked, secreted, and transmembrane proteins can be controlled with high temporal precision in intact animals and cultured organs. We demonstrate the potential of this approach by analyzing the kinetics of ER exit and apical secretion and the spatiotemporal dynamics of tricellular junction assembly in epithelia of living embryos. Furthermore, we show that controllable ER retention enables tissue-specific depletion of secretory protein function. The system is broadly applicable to visualizing and manipulating membrane trafficking in diverse cell types in vivo.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Drosophila / Aparato de Golgi Límite: Animals Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Drosophila / Aparato de Golgi Límite: Animals Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos