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Molecular and physiochemical evaluation of buck semen cryopreserved with antioxidants.
Ghanem, Nasser; Nasr, Norhan Hamdy; Abu Elnaga, Nehal Ali; Abou-Hashim, Fatma; Kamel, Shaimaa; Warda, Mohamad; Dessouki, Sherif M; AbdRabou, Mervat Ahmed; Mehaisen, Gamal M K.
Afiliación
  • Ghanem N; Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt.
  • Nasr NH; Cairo University Research Park, Faculty of Agriculture, Cairo University, Giza, Egypt.
  • Abu Elnaga NA; Zoology and Entomology Department, Faculty of Science, El- Azhar University, Cairo, Egypt.
  • Abou-Hashim F; Zoology and Entomology Department, Faculty of Science, El- Azhar University, Cairo, Egypt.
  • Kamel S; Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt.
  • Warda M; Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
  • Dessouki SM; Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
  • AbdRabou MA; Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt.
  • Mehaisen GMK; Biology Department, College of Science, Jouf University, Saudi Arabia.
Reprod Domest Anim ; 58(6): 813-822, 2023 Jun.
Article en En | MEDLINE | ID: mdl-37013810
The current study evaluated the physiochemical quality and gene expression profile of post-thawed buck semen after supplementation with antioxidants [melatonin (M), L-carnitine (LC), cysteine (Cys), LC + M, M + Cys, LC + Cys, LC + Cys + M] in comparison with the non-treated control group. Physical and biochemical characteristics of semen were evaluated following freezing and thawing. Transcript abundance of six selected candidate genes was profile using quantitative real-time PCR. The data demonstrated significant enhancement of post-freezing total motility, progressive motility, percentage of live sperm, CASA parameters, plasma membrane and acrosome integrity in all groups supplemented with Cys, LC, M + Cys and LC + Cys compared with the control group. The biochemical analysis of semen indicated that semen groups supplemented with LC and LC + Cys recorded increased levels of GPX and SOD that were coupled with up-regulation of antioxidant genes (SOD1, GPX1 and NRF2) and mitochondrial transcripts (CPT2 and ATP5F1A). Moreover, H2O2 level and DNA fragmentation percentage were reduced compared with other groups. In conclusion, supplementation of Cys alone or in combination with LC positively improved the post-thaw physiochemical properties of rabbit semen through activation of bioenergetics-related mitochondrial genes and cellular antioxidant defence mechanism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Preservación de Semen Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Preservación de Semen Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Alemania