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C-Type Natriuretic Peptide Acts as a Microorganism-Activated Regulator of the Skin Commensals Staphylococcus epidermidis and Cutibacterium acnes in Dual-Species Biofilms.
Ovcharova, Maria A; Schelkunov, Mikhail I; Geras'kina, Olga V; Makarova, Nadezhda E; Sukhacheva, Marina V; Martyanov, Sergey V; Nevolina, Ekaterina D; Zhurina, Marina V; Feofanov, Alexey V; Botchkova, Ekaterina A; Plakunov, Vladimir K; Gannesen, Andrei V.
Afiliación
  • Ovcharova MA; Federal Research Centre "Fundamentals of Biotechnology" of Russian Academy of Sciences, Moscow 119071, Russia.
  • Schelkunov MI; Skolkovo Institute of Science and Technology, Moscow 121205, Russia.
  • Geras'kina OV; Institute for Information Transmission Problems of Russian Academy of Sciences, Moscow 127051, Russia.
  • Makarova NE; Biological Faculty, Lomonosov Moscow State University, Moscow 119192, Russia.
  • Sukhacheva MV; Skolkovo Institute of Science and Technology, Moscow 121205, Russia.
  • Martyanov SV; Federal Research Centre "Fundamentals of Biotechnology" of Russian Academy of Sciences, Moscow 119071, Russia.
  • Nevolina ED; Federal Research Centre "Fundamentals of Biotechnology" of Russian Academy of Sciences, Moscow 119071, Russia.
  • Zhurina MV; Federal Research Centre "Fundamentals of Biotechnology" of Russian Academy of Sciences, Moscow 119071, Russia.
  • Feofanov AV; Federal Research Centre "Fundamentals of Biotechnology" of Russian Academy of Sciences, Moscow 119071, Russia.
  • Botchkova EA; Biological Faculty, Lomonosov Moscow State University, Moscow 119192, Russia.
  • Plakunov VK; Federal Research Centre "Fundamentals of Biotechnology" of Russian Academy of Sciences, Moscow 119071, Russia.
  • Gannesen AV; Federal Research Centre "Fundamentals of Biotechnology" of Russian Academy of Sciences, Moscow 119071, Russia.
Biology (Basel) ; 12(3)2023 Mar 12.
Article en En | MEDLINE | ID: mdl-36979128
The effect of C-type natriuretic peptide in a concentration closer to the normal level in human blood plasma was studied on the mono-species and dual-species biofilms of the skin commensal bacteria Cutibacterium acnes HL043PA2 and Staphylococcus epidermidis ATCC14990. Despite the marginal effect of the hormone on cutibacteria in mono-species biofilms, the presence of staphylococci in the community resulted in a global shift of the CNP effect, which appeared to increase the competitive properties of C. acnes, its proliferation and the metabolic activity of the community. S. epidermidis was mostly inhibited in the presence of CNP. Both bacteria had a significant impact on the gene expression levels revealed by RNA-seq. CNP did not affect the gene expression levels in mono-species cutibacterial biofilms; however, in the presence of staphylococci, five genes were differentially expressed in the presence of the hormone, including two ribosomal proteins and metal ABC transporter permease. In staphylococci, the Na-translocating system protein MpsB NADH-quinone oxidoreductase subunit L was downregulated in the dual-species biofilms in the presence of CNP, while in mono-species biofilms, two proteins of unknown function were downregulated. Hypothetically, at least one of the CNP mechanisms of action is via the competition for zinc, at least on cutibacteria.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biology (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biology (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza