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A New Integrative and Mobilizable Element Is a Major Contributor to Tetracycline Resistance in Streptococcus dysgalactiae subsp. equisimilis.
López de Egea, Guillem; González-Díaz, Aida; Guédon, Gérard; Lao, Julie; Berbel, Dàmaris; Casabella, Antonio; Marimón, José María; Cercenado, Emilia; Fernández-Delgado, Lucía; Chiapello, Hélène; Lacroix, Thomas; Domínguez, María Ángeles; Leblond-Bourget, Nathalie; Ardanuy, Carmen.
Afiliación
  • López de Egea G; Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, 08907 L'Hospitalet de Llobregat, Spain.
  • González-Díaz A; Research Network for Respiratory Diseases (CIBERES), Insituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain.
  • Guédon G; Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, 08907 L'Hospitalet de Llobregat, Spain.
  • Lao J; Research Network for Respiratory Diseases (CIBERES), Insituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain.
  • Berbel D; DynAMic, Université de Lorraine, Institut national de recherche pour l'agriculture et l'environnement, Faculté des Sciences et Technologies, F-54000 CEDEX Nancy, France.
  • Casabella A; DynAMic, Université de Lorraine, Institut national de recherche pour l'agriculture et l'environnement, Faculté des Sciences et Technologies, F-54000 CEDEX Nancy, France.
  • Marimón JM; MaIAGE, Université Paris-Saclay, Institut national de recherche pour l'agriculture et l'environnement, Domaine de Vilvert, F-78350 CEDEX Jouy-en-Josas, France.
  • Cercenado E; Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB, 08907 L'Hospitalet de Llobregat, Spain.
  • Fernández-Delgado L; Research Network for Respiratory Diseases (CIBERES), Insituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain.
  • Chiapello H; Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, 08193 Barcelona, Spain.
  • Lacroix T; Microbiology Department, Hospital Universitari Parc Taulí, 08208 Sabadell, Spain.
  • Domínguez MÁ; Biodonostia, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group, Osakidetza Basque Health Service, Donostialdea Integrated Health Organization, Microbiology Department, 20014 San Sebastián, Spain.
  • Leblond-Bourget N; Research Network for Respiratory Diseases (CIBERES), Insituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain.
  • Ardanuy C; Clinical Microbiology and Infectious Disease Department, Hospital General Universitario Gregorio Marañón, 28009 Madrid, Spain.
Antibiotics (Basel) ; 12(3)2023 Mar 15.
Article en En | MEDLINE | ID: mdl-36978446
Tetracycline resistance in streptococci is mainly due to ribosomal protection mediated by the tet(M) gene that is usually located in the integrative and conjugative elements (ICEs) of the Tn916-family. In this study, we analyzed the genes involved in tetracycline resistance and the associated mobile genetic elements (MGEs) in Streptococcus dysgalactiae subsp. equisimilis (SDSE) causing invasive disease. SDSE resistant to tetracycline collected from 2012 to 2019 in a single hospital and from 2018 in three other hospitals were analyzed by whole genome sequencing. Out of a total of 84 SDSE isolates, 24 (28.5%) were resistant to tetracycline due to the presence of tet(M) (n = 22), tet(W) (n = 1), or tet(L) plus tet(W) (n = 1). The tet(M) genes were found in the ICEs of the Tn916-family (n = 10) and in a new integrative and mobilizable element (IME; n = 12). Phylogenetic analysis showed a higher genetic diversity among the strains carrying Tn916 than those having the new IME, which were closely related, and all belonged to CC15. In conclusion, tetracycline resistance in SDSE is mostly due to the tet(M) gene associated with ICEs belonging to the Tn916-family and a new IME. This new IME is a major cause of tetracycline resistance in invasive Streptococcus dysgalactiae subsp. equisimilis in our settings.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Antibiotics (Basel) Año: 2023 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Antibiotics (Basel) Año: 2023 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza