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α-Pinene: Docking Study, Cytotoxicity, Mechanism of Action, and Anti-Biofilm Effect against Candida albicans.
Bomfim de Barros, Daniela; de Oliveira E Lima, Luanna; Alves da Silva, Larissa; Cavalcante Fonseca, Mariana; Ferreira, Rafael Carlos; Diniz Neto, Hermes; da Nóbrega Alves, Danielle; da Silva Rocha, Walicyranison Plinio; Scotti, Luciana; de Oliveira Lima, Edeltrudes; Vieira Sobral, Marianna; Cançado Castellano, Lúcio Roberto; Moura-Mendes, Juliana; Queiroga Sarmento Guerra, Felipe; da Silva, Márcia Vanusa.
Afiliación
  • Bomfim de Barros D; Department of Biochemistry, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
  • de Oliveira E Lima L; Department of Pharmaceutical Sciences, Paraíba Federal University, João Pessoa 58051-900, PB, Brazil.
  • Alves da Silva L; Human Immunology Research and Education Group-GEPIH, Technical School of Health, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil.
  • Cavalcante Fonseca M; Department of Pharmaceutical Sciences, Paraíba Federal University, João Pessoa 58051-900, PB, Brazil.
  • Ferreira RC; Department of Pharmaceutical Sciences, Paraíba Federal University, João Pessoa 58051-900, PB, Brazil.
  • Diniz Neto H; Department of Pharmaceutical Sciences, Paraíba Federal University, João Pessoa 58051-900, PB, Brazil.
  • da Nóbrega Alves D; Postgraduate Program in Natural Products and Synthetic Bioactive, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil.
  • da Silva Rocha WP; University Hospital Julio Muller, Federal University of Mato Grosso, Cuiabá 78060-900, MT, Brazil.
  • Scotti L; Department of Clinical and Social Dentistry, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil.
  • de Oliveira Lima E; Department of Pharmaceutical Sciences, Paraíba Federal University, João Pessoa 58051-900, PB, Brazil.
  • Vieira Sobral M; Cheminformatics Laboratory, Postgraduate Program in Natural Products and Synthetic Bioactive, Quality Management, University Hospital, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil.
  • Cançado Castellano LR; Department of Pharmaceutical Sciences, Paraíba Federal University, João Pessoa 58051-900, PB, Brazil.
  • Moura-Mendes J; Postgraduate Program in Natural Products and Synthetic Bioactive, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil.
  • Queiroga Sarmento Guerra F; Human Immunology Research and Education Group-GEPIH, Technical School of Health, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil.
  • da Silva MV; Centro Multidisciplinario de Investigaciones Tecnológicas, Universidad Nacional de Asunción, San Lorenzo 111421, Paraguay.
Antibiotics (Basel) ; 12(3)2023 Feb 28.
Article en En | MEDLINE | ID: mdl-36978347
Candida albicans is associated with serious infections in immunocompromised patients. Terpenes are natural-product derivatives, widely studied as antifungal alternatives. In a previous study reported by our group, the antifungal activity of α-pinene against C. albicans was verified; α-pinene presented an MIC between 128-512 µg/mL. In this study, we evaluate time-kill, a mechanism of action using in silico and in vitro tests, anti-biofilm activity against the Candida albicans, and toxicity against human cells (HaCaT). Results from the molecular-docking simulation demonstrated that thymidylate synthase (-52 kcal mol-1), and δ-14-sterol reductase (-44 kcal mol-1) presented the best interactions. Our in vitro results suggest that α-pinene's antifungal activity involves binding to ergosterol in the cellular membrane. In the time-kill assay, the antifungal activity was not time-dependent, and also inhibited biofilm formation, while rupturing up to 88% of existing biofilm. It was non-cytotoxic to human keratinocytes. Our study supports α-pinene as a candidate to treat fungal infections caused by C. albicans.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Antibiotics (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Antibiotics (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza