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In-vitro assessment of a novel plant rhizobacterium, Citrobacter freundii, for degrading and biocontrol of food mycotoxin deoxynivalenol.
Murtaza, Bilal; Li, Xiaoyu; Dong, Liming; Saleemi, Muhammad Kashif; Iqbal, Mazhar; Majeed, Saima; Ali, Ashiq; Li, Gen; Jin, Bowen; Wang, Lili; Xu, Yongping.
Afiliación
  • Murtaza B; School of Bioengineering, Dalian University of Technology, Dalian, 116024, China. Electronic address: bilalmurtaza50@gmail.com.
  • Li X; School of Bioengineering, Dalian University of Technology, Dalian, 116024, China; Center for Food Safety of Animal Origin, Ministry of Education, Dalian University of Technology, Dalian, 116600, China.
  • Dong L; Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing, 100048, China.
  • Saleemi MK; Department of Pathology, University of Agriculture, Faisalabad, Pakistan.
  • Iqbal M; National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
  • Majeed S; Institute of Biochemistry, Biotechnology and Bioinformatics (IBBB), The Islamia University, Bahawalpur, 63100, Pakistan.
  • Ali A; Department of Pathology, University of Agriculture, Faisalabad, Pakistan.
  • Li G; School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.
  • Jin B; School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.
  • Wang L; School of Bioengineering, Dalian University of Technology, Dalian, 116024, China; Center for Food Safety of Animal Origin, Ministry of Education, Dalian University of Technology, Dalian, 116600, China.
  • Xu Y; School of Bioengineering, Dalian University of Technology, Dalian, 116024, China; Center for Food Safety of Animal Origin, Ministry of Education, Dalian University of Technology, Dalian, 116600, China. Electronic address: xyping@dlut.edu.cn.
Toxicon ; 227: 107095, 2023 May.
Article en En | MEDLINE | ID: mdl-36972839
Deoxynivalenol (DON) is one of the most harmful and well-known toxins present in food and animal feed throughout the world. Citrobacter freundii (C. freundii-ON077584), a novel DON-degrading strain, was isolated from rice root-linked soil samples. The degrading properties, including DON concentrations, incubation pH, incubation temperatures, bacterial concentrations, and acid treatment effect on degradation, were evaluated. At pH 7 and an incubation temperature of 37 °C, C. freundii demonstrated the capability to degrade more than 90% of DON. The degraded products of DON were identified as 3-keto-DON and DOM-1, which were confirmed by High Performance Liquid Chromatography (HPLC) and Ultra-Performance Liquid Chromatography hyphenated with Tandem Mass Spectrometry (UPLC-MS/MS) analyses. The mechanism of DON degradation into 3-keto-DON and DOM-1 by this bacterial strain will be further explored to identify and purify novel degrading enzymes that can be cloned to the microorganism and added to the animal feed to degrade the DON in the digestion tract.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Micotoxinas Límite: Animals Idioma: En Revista: Toxicon Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Micotoxinas Límite: Animals Idioma: En Revista: Toxicon Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido