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Role of the tyrosine aminotransferase AccTATN gene in the response to pesticide and heavy metal stress in Apis cerana cerana.
Sun, Yunhao; Niu, Xiaojing; Huang, Yuanyuan; Wang, Lijun; Liu, Zhenguo; Guo, Xingqi; Xu, Baohua; Wang, Chen.
Afiliación
  • Sun Y; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, PR China.
  • Niu X; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, PR China.
  • Huang Y; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, PR China.
  • Wang L; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, PR China.
  • Liu Z; College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, PR China.
  • Guo X; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, PR China.
  • Xu B; College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, PR China. Electronic address: bhxu@sdau.edu.cn.
  • Wang C; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, PR China. Electronic address: cwang@sdau.edu.cn.
Pestic Biochem Physiol ; 191: 105372, 2023 Apr.
Article en En | MEDLINE | ID: mdl-36963941
Tyrosine aminotransferase (TATN) is the first enzyme involved in the metabolic degradation of tyrosine, and it plays an important role in tyrosine detoxification and helps the body resist oxidative damage. However, the function of TATN in Apis cerana cerana (A. c. cerana) remains unclear. To explore the role of TATN in the response to pesticide and heavy metal stress in A. c. cerana, AccTATN was isolated and identified. AccTATN was highly expressed in the integument and the adult stage. Exposure to multiple pesticides and heavy metal stress upregulated AccTATN expression. RNA interference experiments showed that silencing AccTATN reduced the resistance of A. c. cerana to glyphosate and avermectins stress. The expression of antioxidant-related genes and the activity of antioxidant enzymes were reduced after AccTATN was silenced, leading to the accumulation of oxidative damage. Overexpression of the recombinant AccTATN protein in a prokaryotic system also confirmed its role in heavy metal stress and improved antioxidant capacity. Our study showed that AccTATN may promote resistance to pesticide and heavy metal stress by regulating the antioxidant capacity of A. c. cerana. This study provides a valuable theoretical basis for A. c. cerana conservation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaguicidas / Antioxidantes Límite: Animals Idioma: En Revista: Pestic Biochem Physiol Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaguicidas / Antioxidantes Límite: Animals Idioma: En Revista: Pestic Biochem Physiol Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos