Your browser doesn't support javascript.
loading
SARS-CoV-2 specific T-cell humoral response assessment after COVID-19 vaccination using a rapid direct real-time PCR amplification.
Cosma, Chiara; Galla, Luisa; Padoan, Andrea; Furlan, Giulia; Marchioro, Lucio; Zaninotto, Martina; Basso, Daniela; Plebani, Mario.
Afiliación
  • Cosma C; Laboratory Medicine Unit, University-Hospital of Padova, Padova, Italy.
  • Galla L; QI.LAB.MED., Spin-off of the University of Padova, Padova, Italy.
  • Padoan A; Laboratory Medicine Unit, University-Hospital of Padova, Padova, Italy.
  • Furlan G; QI.LAB.MED., Spin-off of the University of Padova, Padova, Italy.
  • Marchioro L; Laboratory Medicine Unit, University-Hospital of Padova, Padova, Italy.
  • Zaninotto M; QI.LAB.MED., Spin-off of the University of Padova, Padova, Italy.
  • Basso D; Department of Medicine-DIMED, University-Hospital of Padova, Padova, Italy.
  • Plebani M; QI.LAB.MED., Spin-off of the University of Padova, Padova, Italy.
Clin Chem Lab Med ; 61(9): 1652-1660, 2023 08 28.
Article en En | MEDLINE | ID: mdl-36957995
OBJECTIVES: The SARS-CoV-2 immune response is mediated by both humoral and cellular immunity. In this study, SARS-CoV-2 specific cellular immunity was tested by a novel direct real-time PCR (dRT-PCR) assay, targeting mRNA of CXCL10, and compared with respect to an ELISA measuring interferon gamma (IFN-γ) release. METHODS: Whole blood (Li-He) and serum samples were collected from 92 healthcare workers (HCW), with three doses of homologous (Pfizer/BioNTech, n=74) or heterologous (Pfizer/BioNTech and Vaxzevria or Moderna, n=18) vaccinations. Li-He samples were incubated with SCV2 PANEL-1-T-ACTIVATION (Hyris srl, Lodi, Italy), or CoV-2 IGRA TUBE ELISA (Euroimmune, Lubeck, Germany). CXCL10 mRNA expression was analyzed by bCube/bApp (Hyris), while IFN-γ was evaluated by quant-T-Cell SARS-CoV-2 ELISA (Euroimmune). Anti-SARS-CoV-2 S-RBD IgG levels were measured in sera using a CLIA assay (Snibe, Shenzen, China). RESULTS: Imprecision of dRT-PCR assay was found to be satisfactory, and the two methods for measuring T cell immunity to SARS-CoV-2 peptides agreed in 82/87 (94.2%) of results. At qualitative dRT-PCR analyses, 81 subjects (93.2%) resulted as reactive to SARS-CoV-2 peptides, 3 (3.4%) were borderline and 3 were negative (3.4%). At univariate and multivariate analyses of quantitative dRT-PCR mRNA of CXCL10 and IFN-γ release results showed no difference between HCW with previous infection, homologous/heterologous vaccination, or demographical features. Anti-SARS-CoV-2 S-RBD IgG was associated with the previous infection and the time between the last vaccination or positivity. CONCLUSIONS: Direct RT-PCR appeared accurate for determining the presence or absence of immunoreactivity of SARS-CoV-2 specific T cells, especially when rapid analyses are required, such as for organ transplantation.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T / COVID-19 Tipo de estudio: Diagnostic_studies / Qualitative_research Límite: Humans Idioma: En Revista: Clin Chem Lab Med Asunto de la revista: QUIMICA CLINICA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T / COVID-19 Tipo de estudio: Diagnostic_studies / Qualitative_research Límite: Humans Idioma: En Revista: Clin Chem Lab Med Asunto de la revista: QUIMICA CLINICA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Alemania