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Transcriptomic atlas and interaction networks of brain cells in mouse CNS demyelination and remyelination.
Hou, Jinchao; Zhou, Yingyue; Cai, Zhangying; Terekhova, Marina; Swain, Amanda; Andhey, Prabhakar S; Guimaraes, Rafaela M; Ulezko Antonova, Alina; Qiu, Tian; Sviben, Sanja; Strout, Gregory; Fitzpatrick, James A J; Chen, Yun; Gilfillan, Susan; Kim, Do-Hyun; Van Dyken, Steven J; Artyomov, Maxim N; Colonna, Marco.
Afiliación
  • Hou J; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Zhou Y; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Cai Z; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Terekhova M; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Swain A; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Andhey PS; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Guimaraes RM; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA; Ribeirão Preto Medical School, University of São Paulo - Ribeirão Preto, São Paulo 14049-900, Brazil.
  • Ulezko Antonova A; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Qiu T; Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Sviben S; Washington University Center for Cellular Imaging, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Strout G; Washington University Center for Cellular Imaging, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Fitzpatrick JAJ; Washington University Center for Cellular Imaging, Washington University School of Medicine, St. Louis, MO 63110, USA; Departments of Cell Biology and Physiology and Neuroscience, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Biomedical Engineering, Washington Uni
  • Chen Y; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Gilfillan S; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Kim DH; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Van Dyken SJ; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Artyomov MN; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Colonna M; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address: mcolonna@wustl.edu.
Cell Rep ; 42(4): 112293, 2023 04 25.
Article en En | MEDLINE | ID: mdl-36952346
Demyelination is a hallmark of multiple sclerosis, leukoencephalopathies, cerebral vasculopathies, and several neurodegenerative diseases. The cuprizone mouse model is widely used to simulate demyelination and remyelination occurring in these diseases. Here, we present a high-resolution single-nucleus RNA sequencing (snRNA-seq) analysis of gene expression changes across all brain cells in this model. We define demyelination-associated oligodendrocytes (DOLs) and remyelination-associated MAFBhi microglia, as well as astrocytes and vascular cells with signatures of altered metabolism, oxidative stress, and interferon response. Furthermore, snRNA-seq provides insights into how brain cell types connect and interact, defining complex circuitries that impact demyelination and remyelination. As an explicative example, perturbation of microglia caused by TREM2 deficiency indirectly impairs the induction of DOLs. Altogether, this study provides a rich resource for future studies investigating mechanisms underlying demyelinating diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Desmielinizantes / Remielinización Límite: Animals Idioma: En Revista: Cell Rep Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Desmielinizantes / Remielinización Límite: Animals Idioma: En Revista: Cell Rep Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos