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Examination of the utility of the COVID-19 detection kit, TRC Ready® SARS-CoV-2 i for nasopharyngeal swabs.
Ishii, Satoru; Kimura, Moto; Miyoshi-Akiyama, Tohru; Moriya, Ataru; Kurokawa, Masami; Isaka, Erina; Terada-Hirashima, Junko; Takasaki, Jin; Izumi, Shinyu; Hojo, Masayuki; Sugiyama, Haruhito.
Afiliación
  • Ishii S; Department of Respiratory Medicine, National Center for Global Health and Medicine, Tokyo, Japan.
  • Kimura M; Center for Clinical Sciences, National Center for Global Health and Medicine, Tokyo, Japan.
  • Miyoshi-Akiyama T; Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
  • Moriya A; Laboratory Testing Department, National Center for Global Health and Medicine, Tokyo, Japan.
  • Kurokawa M; Laboratory Testing Department, National Center for Global Health and Medicine, Tokyo, Japan.
  • Isaka E; Laboratory Testing Department, National Center for Global Health and Medicine, Tokyo, Japan.
  • Terada-Hirashima J; Department of Respiratory Medicine, National Center for Global Health and Medicine, Tokyo, Japan.
  • Takasaki J; Department of Respiratory Medicine, National Center for Global Health and Medicine, Tokyo, Japan.
  • Izumi S; Department of Respiratory Medicine, National Center for Global Health and Medicine, Tokyo, Japan.
  • Hojo M; Department of Respiratory Medicine, National Center for Global Health and Medicine, Tokyo, Japan.
  • Sugiyama H; Department of Respiratory Medicine, National Center for Global Health and Medicine, Tokyo, Japan.
Drug Discov Ther ; 17(2): 134-138, 2023 May 15.
Article en En | MEDLINE | ID: mdl-36948642
The reverse transcription polymerase chain reaction (RT-PCR) offers high sensitivity, but has some drawbacks, such as the time required for the RNA extraction. Transcription reverse-transcription concerted reaction (TRC) Ready® SARS-CoV-2 i is easy to use and can be performed in about 40 minutes. TRC Ready® SARS-CoV-2 i and real-time one-step RT-PCR using the TaqMan probe tests of cryopreserved nasopharyngeal swab samples from patients diagnosed with COVID-19 were compared. The primary objective was to examine the positive and negative concordance rates. A total of 69 samples cryopreserved at -80° C were examined. Of the 37 frozen samples that were expected to be RT-PCR positive, 35 were positive by the RT-PCR method. TRC Ready® SARS-CoV-2 i detected 33 positive cases and 2 negative cases. One frozen sample that was expected to be RT-PCR positive was negative on both TRC Ready® SARS-CoV-2 i and RT-PCR. In addition, one frozen sample that was expected to be RT-PCR positive was positive by the RT-PCR method and negative by TRC Ready® SARS-CoV-2 i. Of the 32 frozen samples that were expected to be RT-PCR negative, both the RT-PCR method and TRC Ready® SARS-CoV-2 i yielded negative results for all 32 samples. Compared with RT-PCR, TRC Ready® SARS-CoV-2 i had a positive concordance rate of 94.3% and a negative concordance rate of 97.1%. TRC Ready® SARS-CoV-2 i can be utilized in a wide range of medical sites such as clinics and community hospitals due to its ease of operability, and is expected to be useful in infection control.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: COVID-19 Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Drug Discov Ther Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: COVID-19 Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Drug Discov Ther Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón