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Homocysteine exchange across skeletal muscle in patients with chronic kidney disease.
Garibotto, Giacomo; Picciotto, Daniela; Verzola, Daniela; Valli, Alessando; Sofia, Antonella; Costigliolo, Francesca; Saio, Michela; Viazzi, Francesca; Esposito, Pasquale.
Afiliación
  • Garibotto G; Department of Internal Medicine, University of Genova, Genova, Italy.
  • Picciotto D; Division of Nephrology, Dialysis and Transplantation, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
  • Verzola D; Department of Internal Medicine, University of Genova, Genova, Italy.
  • Valli A; Department of Internal Medicine, University of Genova, Genova, Italy.
  • Sofia A; Division of Nephrology, Dialysis and Transplantation, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
  • Costigliolo F; Division of Nephrology, Dialysis and Transplantation, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
  • Saio M; Division of Nephrology, Dialysis and Transplantation, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
  • Viazzi F; Department of Internal Medicine, University of Genova, Genova, Italy.
  • Esposito P; Division of Nephrology, Dialysis and Transplantation, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
Physiol Rep ; 11(6): e15573, 2023 03.
Article en En | MEDLINE | ID: mdl-36945836
Sites and mechanisms regulating the supply of homocysteine (Hcy) to the circulation are unexplored in humans. We studied the exchange of Hcy across the forearm in CKD patients (n = 17, eGFR 20 ± 2 ml/min), in hemodialysis (HD)-treated patients (n = 14) and controls (n = 9). Arterial Hcy was ~ 2.5 folds increased in CKD and HD patients (p < 0.05-0.03 vs. controls). Both in controls and in patients Hcy levels in the deep forearm vein were consistently greater (+~7%, p < 0.05-0.01) than the corresponding arterial levels, indicating the occurrence of Hcy release from muscle. The release of Hcy from the forearm was similar among groups. In all groups arterial Hcy varied with its release from muscle (p < 0.03-0.02), suggesting that muscle plays an important role on plasma Hcy levels. Forearm Hcy release was inversely related to folate plasma level in all study groups but neither to vitamin B12 and IL-6 levels nor to muscle protein net balance. These data indicate that the release of Hcy from peripheral tissue metabolism plays a major role in influencing its Hcy plasma levels in humans and patients with CKD, and that folate is a major determinant of Hcy release.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Insuficiencia Renal Crónica Límite: Humans Idioma: En Revista: Physiol Rep Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Insuficiencia Renal Crónica Límite: Humans Idioma: En Revista: Physiol Rep Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos