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Single-nucleus RNA sequencing unveils critical regulators in various hippocampal neurons for anti-N-methyl-D-aspartate receptor encephalitis.
Bai, Yunmeng; Liu, Zhuhe; Qian, Tinglin; Peng, Yu; Ma, Huan; Hu, Hong; Cheng, Guangqing; Wen, Haixia; Xie, Lulin; Zheng, Dong; Geng, Qingshan; Wang, Jigang; Wang, Honghao.
Afiliación
  • Bai Y; Department of Nephrology, Shenzhen Key Laboratory of Kidney Diseases, Shenzhen People's Hospital, the First Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, China.
  • Liu Z; Department of Neurology, Guangzhou First People's Hospital, School of Medicine, Southern China University of Technology, Guangzhou, China.
  • Qian T; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
  • Peng Y; Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Ma H; Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou, China.
  • Hu H; Department of Nephrology, Shenzhen Key Laboratory of Kidney Diseases, Shenzhen People's Hospital, the First Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, China.
  • Cheng G; Department of Nephrology, Shenzhen Key Laboratory of Kidney Diseases, Shenzhen People's Hospital, the First Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, China.
  • Wen H; Department of Neurology, Guangzhou First People's Hospital, School of Medicine, Southern China University of Technology, Guangzhou, China.
  • Xie L; Department of Nephrology, Shenzhen Key Laboratory of Kidney Diseases, Shenzhen People's Hospital, the First Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, China.
  • Zheng D; Department of Neurology, The Affiliated Brain Hospital of Guangzhou Medical University, Guangzhou, China.
  • Geng Q; Department of Nephrology, Shenzhen Key Laboratory of Kidney Diseases, Shenzhen People's Hospital, the First Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, China.
  • Wang J; Department of Nephrology, Shenzhen Key Laboratory of Kidney Diseases, Shenzhen People's Hospital, the First Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, China.
  • Wang H; Department of Neurology, Guangzhou First People's Hospital, School of Medicine, Southern China University of Technology, Guangzhou, China.
Brain Pathol ; 33(4): e13156, 2023 07.
Article en En | MEDLINE | ID: mdl-36942475
Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is a neuropsychiatric disease with variable clinical manifestations caused by NMDAR autoantibody. The underlying molecular underpinnings of this disease are rarely characterized on a genomic scale. Anti-NMDAR encephalitis mainly affects the hippocampus, however, its effect on gene expression in hippocampal neurons is unclear at present. Here, we construct the active and passive immunization mouse models of anti-NMDAR encephalitis, and use single-nucleus RNA sequencing to investigate the diverse expression profile of neuronal populations isolated from different hippocampal regions. Dramatic changes in cell proportions and differentially expressed genes were observed in excitatory neurons of the dentate gyrus (DG) subregion. In addition, we found that ATP metabolism and biosynthetic regulators related genes in excitatory neurons of DG subregion were significantly affected. Kcnq1ot1 in inhibitory neurons and Meg3 in interneurons also changed. Notably, the latter two molecules exhibited opposite changes in different models. Therefore, the above genes were used as potential targets for further research on the pathological process of anti-NMDAR encephalitis. These data involve various hippocampal neurons, which delineate a framework for understanding the hippocampal neuronal circuit and the potential molecular mechanisms of anti-NMDAR encephalitis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encefalitis Antirreceptor N-Metil-D-Aspartato Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Brain Pathol Asunto de la revista: CEREBRO / PATOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encefalitis Antirreceptor N-Metil-D-Aspartato Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Brain Pathol Asunto de la revista: CEREBRO / PATOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza