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USP14 Regulates ATF2/PIK3CD Axis to Promote Microvascular Endothelial Cell Proliferation, Migration, and Angiogenesis in Diabetic Retinopathy.
He, Fu-Tao; Fu, Xiao-Lin; Li, Mo-Han; Fu, Chun-Yan; Chen, Jian-Zhi.
Afiliación
  • He FT; Department of Ophthalmology, Hainan West Central Hospital, No.2 Fubo East Road, Nada Town, Danzhou, 571700, Hainan Province, People's Republic of China.
  • Fu XL; Department of Ophthalmology, Hainan West Central Hospital, No.2 Fubo East Road, Nada Town, Danzhou, 571700, Hainan Province, People's Republic of China. xiaolinfu12345@163.com.
  • Li MH; Department of Ophthalmology, Hainan West Central Hospital, No.2 Fubo East Road, Nada Town, Danzhou, 571700, Hainan Province, People's Republic of China.
  • Fu CY; Department of Ophthalmology, Hainan West Central Hospital, No.2 Fubo East Road, Nada Town, Danzhou, 571700, Hainan Province, People's Republic of China.
  • Chen JZ; Department of Ophthalmology, Hainan West Central Hospital, No.2 Fubo East Road, Nada Town, Danzhou, 571700, Hainan Province, People's Republic of China.
Biochem Genet ; 61(5): 2076-2091, 2023 Oct.
Article en En | MEDLINE | ID: mdl-36939972
Diabetic retinopathy (DR) is one of the leading causes of blindness in diabetic patients. However, the pathogenesis of DR is complex, and no firm conclusions have been drawn so far. It has become a hot spot in ophthalmology research to deeply study the mechanism of DR pathological changes and find effective treatment options. Human retinal microvascular endothelial cells (HRMECs) were induced by high glucose (HG) to construct DR cell model. CCK-8 assay was used to detect the viability of HRMECs. Transwell assay was used to detect the migration ability of HRMECs. Tube formation assay was used to identify the tube formation ability of HRMECs. The expressions of USP14, ATF2 and PIK3CD were detected by Western blot analysis and qRT-PCR assay. Immunoprecipitation (IP) was used to ascertain the relationship of USP14 and ATF2. To explore the regulatory relationship between ATF2 and PIK3CD by dual-luciferase reporter gene assay and Chromatin immunoprecipitation (ChIP) assay. High glucose treatment promoted the proliferation, migration, and tube formation of HRMEC, and the expressions of USP14, ATF2 and PIK3CD were significantly up-regulated. USP14 or ATF2 knockdown inhibited HG-induced HRMECs proliferation, migration, and tube formation. USP14 regulated the expression of ATF2, and ATF2 promoted PIK3CD expression. PIK3CD overexpression attenuated the inhibitory effectiveness of USP14 knockdown on proliferation, migration and tube formation of DR cell model. Here, we revealed that USP14 regulated the ATF2/PIK3CD axis to promote proliferation, migration, and tube formation in HG-induced HRMECs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Diabetes Mellitus / Retinopatía Diabética Límite: Humans Idioma: En Revista: Biochem Genet Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Diabetes Mellitus / Retinopatía Diabética Límite: Humans Idioma: En Revista: Biochem Genet Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos