Isolation and antigenic reactivity of Brucella ovis outer membrane proteins.
J Clin Microbiol
; 25(11): 2132-5, 1987 Nov.
Article
en En
| MEDLINE
| ID: mdl-3693544
Brucella ovis cell membranes were isolated from fractured and lysozyme-treated cells by ultracentrifugation. These preparations appeared to consist largely of outer membranes, as judged from the results of ultracentrifugation experiments in sucrose density gradients under conditions that are widely used to separate inner and outer membranes of gram-negative bacteria. The sequential detergent extraction of cell membranes yielded mainly lipopolysaccharide and three groups of outer membrane proteins. In immunoblotting, lipopolysaccharide had good antigenic reactivity with all sera from rams exposed to B. ovis (vaccination or natural infection), but some outer membrane proteins reacted strongly only with sera from immune (vaccinated) rams, not from infected rams, suggesting a possible diagnostic role for such proteins in predicting immunity or infection.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de la Membrana Bacteriana Externa
/
Brucella
/
Antígenos Bacterianos
Límite:
Animals
Idioma:
En
Revista:
J Clin Microbiol
Año:
1987
Tipo del documento:
Article
Pais de publicación:
Estados Unidos