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A deficiency screen of the 3rd chromosome for dominant modifiers of the Drosophila ER integral membrane protein, Jagunal.
Ascencio, Gerson; de Cruz, Matthew A; Abuel, Judy; Alvarado, Sydney; Arriaga, Yuma; Conrad, Emily; Castro, Alonso; Eichelberger, Katharine; Galvan, Laura; Gundy, Grace; Garcia, Jorge Alberto Inojoza; Jimenez, Alyssa; Lu, Nhein Tuyet; Lugar, Catharine; Marania, Ronald; Mendsaikhan, Tserendavaa; Ortega, Jose; Nand, Natasha; Rodrigues, Nicole S; Shabazz, Khayla; Tam, Cynnie; Valenciano, Emmanuel; Hayzelden, Clive; Eritano, Anthony S; Riggs, Blake.
Afiliación
  • Ascencio G; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • de Cruz MA; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Abuel J; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Alvarado S; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Arriaga Y; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Conrad E; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Castro A; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Eichelberger K; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Galvan L; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Gundy G; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Garcia JAI; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Jimenez A; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Lu NT; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Lugar C; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Marania R; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Mendsaikhan T; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Ortega J; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Nand N; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Rodrigues NS; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Shabazz K; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Tam C; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Valenciano E; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Hayzelden C; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Eritano AS; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
  • Riggs B; Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA 4132, USA.
G3 (Bethesda) ; 13(7)2023 07 05.
Article en En | MEDLINE | ID: mdl-36932646
The mechanism surrounding chromosome inheritance during cell division has been well documented, however, organelle inheritance during mitosis is less understood. Recently, the endoplasmic reticulum (ER) has been shown to reorganize during mitosis, dividing asymmetrically in proneuronal cells prior to cell fate selection, indicating a programmed mechanism of inheritance. ER asymmetric partitioning in proneural cells relies on the highly conserved ER integral membrane protein, Jagunal (Jagn). Knockdown of Jagn in the compound Drosophila eye displays a pleotropic rough eye phenotype in 48% of the progeny. To identify genes involved in Jagn dependent ER partitioning pathway, we performed a dominant modifier screen of the 3rd chromosome for enhancers and suppressors of this Jagn-RNAi-induced rough eye phenotype. We screened through 181 deficiency lines covering the 3L and 3R chromosomes and identified 12 suppressors and 10 enhancers of the Jagn-RNAi phenotype. Based on the functions of the genes covered by the deficiencies, we identified genes that displayed a suppression or enhancement of the Jagn-RNAi phenotype. These include Division Abnormally Delayed (Dally), a heparan sulfate proteoglycan, the γ-secretase subunit Presenilin, and the ER resident protein Sec63. Based on our understanding of the function of these targets, there is a connection between Jagn and the Notch signaling pathway. Further studies will elucidate the role of Jagn and identified interactors within the mechanisms of ER partitioning during mitosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Drosophila / Drosophila Límite: Animals Idioma: En Revista: G3 (Bethesda) Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Drosophila / Drosophila Límite: Animals Idioma: En Revista: G3 (Bethesda) Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido