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Extracellular Matrix-Derived Biophysical Cues Mediate Interstitial Flow-Induced Sprouting Angiogenesis.
Chang, Chia-Wen; Shih, Hsiu-Chen; Cortes-Medina, Marcos G; Beshay, Peter E; Avendano, Alex; Seibel, Alex J; Liao, Wei-Hao; Tung, Yi-Chung; Song, Jonathan W.
Afiliación
  • Chang CW; Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, United States.
  • Shih HC; Research Center for Applied Science, Academia Sinica, Taipei 115-29, Taiwan.
  • Cortes-Medina MG; Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, United States.
  • Beshay PE; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, Ohio 43210, United States.
  • Avendano A; Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, United States.
  • Seibel AJ; Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, United States.
  • Liao WH; Research Center for Applied Science, Academia Sinica, Taipei 115-29, Taiwan.
  • Tung YC; Research Center for Applied Science, Academia Sinica, Taipei 115-29, Taiwan.
  • Song JW; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, Ohio 43210, United States.
ACS Appl Mater Interfaces ; 15(12): 15047-15058, 2023 Mar 29.
Article en En | MEDLINE | ID: mdl-36916875
Sprouting angiogenesis is orchestrated by an intricate balance of biochemical and mechanical cues in the local tissue microenvironment. Interstitial flow has been established as a potent regulator of angiogenesis. Similarly, extracellular matrix (ECM) physical properties, such as stiffness and microarchitecture, have also emerged as important mediators of angiogenesis. However, the interplay between interstitial flow and ECM physical properties in the initiation and control of angiogenesis is poorly understood. Using a three-dimensional (3D) microfluidic tissue analogue of angiogenic sprouting with defined interstitial flow superimposed over ECM with well-characterized physical properties, we found that the addition of hyaluronan (HA) to collagen-based matrices significantly enhances sprouting induced by interstitial flow compared to responses in collagen-only hydrogels. We confirmed that both the stiffness and matrix pore size of collagen-only hydrogels were increased by the addition of HA. Interestingly, interstitial flow-potentiated sprouting responses in collagen/HA matrices were not affected when functionally blocking the HA receptor CD44. In contrast, enzymatic depletion of HA in collagen/HA matrices with hyaluronidase (HAdase) resulted in decreased stiffness, pore size, and interstitial flow-mediated sprouting to the levels observed in collagen-only matrices. Taken together, these results suggest that HA enhances interstitial flow-mediated angiogenic sprouting through its alterations to collagen ECM stiffness and pore size.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Señales (Psicología) / Matriz Extracelular Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Señales (Psicología) / Matriz Extracelular Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos