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Effects of combining flow intermittency and exposure to emerging contaminants on the composition and metabolic response of streambed biofilm bacterial communities.
Rozman, Marko; Lekunberri, Itziar; Grgic, Ivana; Borrego, Carles M; Petrovic, Mira.
Afiliación
  • Rozman M; Ruder Boskovic Institute, Bijenicka cesta 54, 10000 Zagreb, Croatia; Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona, Spain. Electronic address: marko.rozman@irb.hr.
  • Lekunberri I; Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona, Spain.
  • Grgic I; Ruder Boskovic Institute, Bijenicka cesta 54, 10000 Zagreb, Croatia.
  • Borrego CM; Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona, Spain; Group of Molecular Microbial Ecology, Institute of Aquatic Ecology, University of Girona, E-17001 Girona, Spain.
  • Petrovic M; Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona, Spain; Catalan Institution for Research and Advanced Studies (ICREA), Passeig Lluís Companys 23, 08010 Barcelona, Spain.
Sci Total Environ ; 877: 162818, 2023 Jun 15.
Article en En | MEDLINE | ID: mdl-36914121
Freshwater ecosystems are characterised by the co-occurrence of stressors that simultaneously affect the biota. Among these, flow intermittency and chemical pollution severely impair the diversity and functioning of streambed bacterial communities. Using an artificial streams mesocosm facility, this study examined how desiccation and pollution caused by emerging contaminants affect the composition of stream biofilm bacterial communities, their metabolic profiles, and interactions with their environment. Through an integrative analysis of the composition of biofilm communities, characterization of their metabolome and composition of the dissolved organic matter, we found strong genotype-to-phenotype interconnections. The strongest correlation was found between the composition and metabolism of the bacterial community, both of which were influenced by incubation time and desiccation. Unexpectedly, no effect of the emerging contaminants was observed, which was due to the low concentration of the emerging contaminants and the dominant impact of desiccation. However, biofilm bacterial communities modified the chemical composition of their environment under the effect of pollution. Considering the tentatively identified classes of metabolites, we hypothesised that the biofilm response to desiccation was mainly intracellular while the response to chemical pollution was extracellular. The present study demonstrates that metabolite and dissolved organic matter profiling may be effectively integrated with compositional analysis of stream biofilm communities to yield a more complete picture of changes in response to stressors.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Ecosistema Idioma: En Revista: Sci Total Environ Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Ecosistema Idioma: En Revista: Sci Total Environ Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos