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Chemical, structural and cytotoxicity characterisation of experimental fluoride-doped calcium phosphates as promising remineralising materials for dental applications.
Sauro, Salvatore; Spagnuolo, Gianrico; Del Giudice, Carmela; Neto, Davino M Andrade; Fechine, Pierre B A; Chen, Xiaohui; Rengo, Sandro; Chen, Xiaojing; Feitosa, Victor P.
Afiliación
  • Sauro S; Dental Biomaterials & Minimally Invasive Dentistry, Departamento de Odontologia, CEU Cardenal Herrera University, Alfara del Patriarca, Valencia, Spain. Electronic address: salvatore.sauro@uch.ceu.es.
  • Spagnuolo G; Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples "Federico II", 80131 Naples, Italy.
  • Del Giudice C; Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples "Federico II", 80131 Naples, Italy.
  • Neto DMA; Federal Institute of Education, Science, and Technology of Ceará, Campus Camocim, 62400-000 Camocim, CE, Brazil.
  • Fechine PBA; Grupo de Química de Materiais Avançados (GQMat) - Departamento de Química Analítica e Físico-Química, Universidade Federal do Ceará (UFC), Campus do Pici, CP 12100, Fortaleza CE 60451-970, Brazil.
  • Chen X; Division of Dentistry, School of Medical Sciences, The University of Manchester, Manchester, UK.
  • Rengo S; Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples "Federico II", 80131 Naples, Italy.
  • Chen X; Xiangya School of Stomatology and Hospital, Central South University, Changsha 410008, Hunan, China; Hunan Key Laboratory of Oral Health Research, Central South University, Changsha, 410008, Hunan, China; Institute of Dentistry, Dental Physical Sciences Unit, Queen Mary University of London, London,
  • Feitosa VP; Research Division, Paulo Picanço School of Dentistry, 900 Joaquim Sá St., Fortaleza 60135-218, Ceará, Brazil.
Dent Mater ; 39(4): 391-401, 2023 04.
Article en En | MEDLINE | ID: mdl-36907820
OBJECTIVES: This study aimed at evaluating the cytotoxicity, chemical and structural properties of experimental fluoride-doped calcium-phosphates as potential remineralising materials for dental applications. METHODS: Experimental calcium phosphates were formulated using ß-tricalcium phosphate, monocalcium phosphate monohydrate, calcium hydroxide, and different concentrations of calcium/sodium fluoride salts [(5 wt%: VSG5F), (10 wt%: VSG10F), (20 wt%: VSG20F)]. A fluoride-free calcium phosphate (VSG) was used as control. Each tested material was immersed in simulated body fluid (SBF), (24 h, 15 and 30 days) to assess their ability to crystallise into apatite-like. Cumulative fluoride release was assayed up to 45 days. Moreover, each powder was placed into a medium containing human dental pulp stem cells (200 mg/mL) and their cytotoxicity was analysed using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay (24 h, 48 h and 72 h incubation). These latter results were statistically analysed by ANOVA and Tukey's test (α = 0.05). RESULTS: All the experimental VSG-F materials produced fluoride-containing apatite-like crystals after SBF immersion. VSG20F presented prolonged release of fluoride ions into the storage media (45d). VSG, VSG10F and VSG20F showed a significant cytotoxicity at dilution of 1:1, while at 1:5, only VSG and VSG20F demonstrated a reduction in cell viability. At lower dilutions (1:10, 1:50 and 1:100) all specimens showed no significant toxicity to hDPSCs, but an increase in cell proliferation. SIGNIFICANCE: The experimental fluoride-doped calcium-phosphates are biocompatible and possess a clear ability to evoke fluoride-containing apatite-like crystallisation. Hence, they may be promising remineralising materials for dental applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatos / Calcio Límite: Humans Idioma: En Revista: Dent Mater Asunto de la revista: ODONTOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatos / Calcio Límite: Humans Idioma: En Revista: Dent Mater Asunto de la revista: ODONTOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido