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Cloning of STC-1 and analysis of its differential expression in Hezuo pig.
Yan, Jihong; Wang, Pengfei; Yan, Zunqiang; Yang, Qiaoli; Huang, Xiaoyu; Gao, Xiaoli; Li, Jiyou; Wang, Zike; Gao, Yi; Gun, Shuangbao.
Afiliación
  • Yan J; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
  • Wang P; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
  • Yan Z; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
  • Yang Q; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
  • Huang X; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
  • Gao X; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
  • Li J; Gansu General Station of Animal Husbandry Technology Extension, Lanzhou, China.
  • Wang Z; Gansu General Station of Animal Husbandry Technology Extension, Lanzhou, China.
  • Gao Y; Jilin Rongtai Agricultural Development Co., Ltd., Changchun, China.
  • Gun S; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
Anim Biotechnol ; 34(9): 4687-4694, 2023 Dec.
Article en En | MEDLINE | ID: mdl-36905141
The purpose of this study was to examine STC-1's structure, function, and differential expression in large and miniature pigs. We cloned the Hezuo pig's coding sequence, compared its homology, and used bioinformatics to assess the structure. RT-qPCR and Western blot were used to detect the expression in ten tissues of Hezuo pig and Landrace pig. The results showed that Hezuo pig was most closely related to Capra hircus and most distantly related to Danio rerio. The protein STC-1 has a signal peptide and its secondary structure is dominated by the alpha helix. The mRNA expression in the spleen, duodenum, jejunum, and stomach of Hezuo pigs was higher than that of Landrace pigs. And except for heart and duodenum, expression of the protein in Hezuo pig was higher than in another. In conclusion, STC-1 is highly conserved among different breeds of pigs, and the expression and distribution of its mRNA and protein are different in large and miniature pigs. This work can lay the foundation for future study into the mechanism of action of STC-1 in Hezuo pigs and the enhancement of breeding in miniature pigs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clonación de Organismos Límite: Animals Idioma: En Revista: Anim Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clonación de Organismos Límite: Animals Idioma: En Revista: Anim Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido