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Dihydroartemisinin Affects STAT3/DDA1 Signaling Pathway and Reverses Breast Cancer Resistance to Cisplatin.
Zhang, Jing; Li, Yang; Wang, Ji-Guo; Feng, Jing-Yu; Huang, Guo-Dong; Luo, Chang-Guo.
Afiliación
  • Zhang J; The First School of Medicine, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China.
  • Li Y; Department of Oncology, Baoan Hospital of Traditional Chinese Medicine, Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, No. 25, Yu'an Second Road, Bao'an District, Shenzhen 518133, Guangdong Province, P. R. China.
  • Wang JG; Department of Oncology, Baoan Hospital of Traditional Chinese Medicine, Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, No. 25, Yu'an Second Road, Bao'an District, Shenzhen 518133, Guangdong Province, P. R. China.
  • Feng JY; Department of Oncology, Baoan Hospital of Traditional Chinese Medicine, Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, No. 25, Yu'an Second Road, Bao'an District, Shenzhen 518133, Guangdong Province, P. R. China.
  • Huang GD; Department of Oncology, Baoan Hospital of Traditional Chinese Medicine, Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, No. 25, Yu'an Second Road, Bao'an District, Shenzhen 518133, Guangdong Province, P. R. China.
  • Luo CG; Department of Oncology, Baoan Hospital of Traditional Chinese Medicine, Affiliated to Guangzhou University of Chinese Medicine, Shenzhen, No. 25, Yu'an Second Road, Bao'an District, Shenzhen 518133, Guangdong Province, P. R. China.
Am J Chin Med ; 51(2): 445-459, 2023.
Article en En | MEDLINE | ID: mdl-36891981
Dihydroartemisinin (DHA) has anticancer effects on multiple tumors, including those associated with breast cancer. This study aimed to investigate the mechanism causing DHA-reversing cisplatin (DDP) resistance in breast cancer. Relative mRNA and protein levels were tested using a qRT-PCR and western blot assay. Cell proliferation, viability, and apoptosis were evaluated using colony formation, MTT, and flow cytometry assays, respectively. Interaction of STAT3 and DDA1 was measured via a dual-luciferase reporter assay. The results showed that DDA1 and p-STAT3 levels were dramatically elevated in DDP-resistant cells. DHA treatment repressed proliferation and induced apoptosis of DDP-resistant cells by suppressing STAT3 phosphorylation; the inhibition ability was positively proportional to the DHA concentration. DDA1 knockdown inhibited cyclin expression, promoted G0/G1 phase arrest, restrained cell proliferation, and induced apoptosis of DDP-resistant cells. Furthermore, knockdown of STAT3 restrained proliferation and induced apoptosis and G0/G1 cell cycle arrest of DDP-resistant cells by targeting DDA1. DHA could restrain tumor proliferation of breast cancer via enhancing drug sensitivity of DDP-resistant cells through the STAT3/DDA1 signaling pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Neoplasias de la Mama / MicroARNs / Antineoplásicos Límite: Female / Humans Idioma: En Revista: Am J Chin Med Año: 2023 Tipo del documento: Article Pais de publicación: Singapur
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Neoplasias de la Mama / MicroARNs / Antineoplásicos Límite: Female / Humans Idioma: En Revista: Am J Chin Med Año: 2023 Tipo del documento: Article Pais de publicación: Singapur