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Piezo1-mediated M2 macrophage mechanotransduction enhances bone formation through secretion and activation of transforming growth factor-ß1.
Cai, Guanhui; Lu, Yahui; Zhong, Weijie; Wang, Ting; Li, Yingyi; Ruan, Xiaolei; Chen, Hongyu; Sun, Lian; Guan, Zhaolan; Li, Gen; Zhang, Hengwei; Sun, Wen; Chen, Minglong; Zhang, Wei-Bing; Wang, Hua.
Afiliación
  • Cai G; Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
  • Lu Y; Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.
  • Zhong W; Department of Stomatology, Dushu Lake Hospital Affiliated to Soochow University, Soochow, China.
  • Wang T; Department of Stomatology, Medical Center of Soochow University, Soochow, China.
  • Li Y; Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
  • Ruan X; Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
  • Chen H; Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
  • Sun L; Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
  • Guan Z; Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
  • Li G; Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
  • Zhang H; Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
  • Sun W; Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
  • Chen M; Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA.
  • Zhang WB; Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
  • Wang H; Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
Cell Prolif ; 56(9): e13440, 2023 Sep.
Article en En | MEDLINE | ID: mdl-36880296
Macrophages are multifunctional immune system cells that are essential for the mechanical stimulation-induced control of metabolism. Piezo1 is a non-selective calcium channel expressed in multifarious tissues to convey mechanical signals. Here, a cellular model of tension was used to study the effect of mechanical stretch on the phenotypic transformation of macrophages and its mechanism. An indirect co-culture system was used to explore the effect of macrophage activation on bone marrow mesenchymal stem cells (BMSCs), and a treadmill running model was used to validate the mechanism in vivo for in vitro studies. p53 was acetylated and deacetylated by macrophages as a result of mechanical strain being detected by Piezo1. This process is able to polarize macrophages towards M2 and secretes transforming growth factor-beta (TGF-ß1), which subsequently stimulates BMSCs migration, proliferation and osteogenic differentiation. Knockdown of Piezo1 inhibits the conversion of macrophages to the reparative phenotype, thereby affecting bone remodelling. Blockade of TGF-ß I, II receptors and Piezo1 significantly reduced exercise-increased bone mass in mice. In conclusion, we showed that mechanical tension causes calcium influx, p53 deacetylation, macrophage polarization towards M2 and TGF-ß1 release through Piezo1. These events support BMSC osteogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Factor de Crecimiento Transformador beta1 Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Cell Prolif Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Factor de Crecimiento Transformador beta1 Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Cell Prolif Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido