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miR-330-3p alleviates the progression of atherosclerosis by downregulating AQP9.
Shan, Erbo; Yu, Yuanyuan; Tang, Wenbo; Wang, Wei; Wang, Xiangkui; Zhou, Shaobo; Gao, Yong.
Afiliación
  • Shan E; The First Affiliated Hospital of Jinan University, Guangzhou, China.
  • Yu Y; The Second Affiliated Hospital of Bengbu Medical College, Bengbu, China.
  • Tang W; The Second Affiliated Hospital of Bengbu Medical College, Bengbu, China.
  • Wang W; Department of Vascular Surgery, the First Affiliated Hospital of Bengbu Medical College, No. 287 Changhuai Road, Bengbu, 233004, China.
  • Wang X; The Second Affiliated Hospital of Bengbu Medical College, Bengbu, China.
  • Zhou S; Department of Vascular Surgery, Huaibei General Miner Hospital, Huaibei, China.
  • Gao Y; The Second Affiliated Hospital of Bengbu Medical College, Bengbu, China.
Funct Integr Genomics ; 23(2): 77, 2023 Mar 07.
Article en En | MEDLINE | ID: mdl-36879069
Atherosclerosis (AS) is the main cause of cardiovascular diseases. However, the role of AQP9 in AS is not well understood. In the present study, we predicted that miR-330-3p might regulate AQP9 in AS through bioinformatics analysis, and we established AS model using ApoE-/- mouse (C57BL/6) with high-fat diet (HFD). Hematoxylin and eosin (H&E) and Oil red O staining were used to determine atherosclerotic lesions. CCK8 and Ethyny1-2-deoxyuridine (EdU) assays were used to investigate human umbilical vein endothelial cells (HUVECs) proliferation after treatment with 100 µg/mL ox-LDL. Wound scratch healing and transwell assays were used to measure the cell invasion and migration ability. Flow cytometry assay was used to determine apoptosis and cell cycle. A dual-luciferase reporter assay was performed to investigate the binding of miR-330-3p and AQP9. We identified that the expression of miR-330-3p in AS mice model decreased while the expression level of AQP9 increased. miR-330-3p overexpression or down-regulation of AQP9 could reduce cell apoptosis, promote cell proliferation, and migration after ox-LDL treatment. Dual-luciferase reporter assay result presented that AQP9 was directly inhibited by miR-330-3p. These results suggest that miR-330-3p inhibits AS by regulating AQP9. miR-330-3p/AQP9 axis may be a new therapeutic target for AS.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acuaporinas / MicroARNs / Aterosclerosis Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Funct Integr Genomics Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acuaporinas / MicroARNs / Aterosclerosis Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Funct Integr Genomics Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania