Super-resolution imaging of synaptic scaffold proteins in rat hippocampal neurons.

Guzikowski, Natalie J; Kavalali, Ege T

Guzikowski, Natalie J; Kavalali, Ege T.

Afiliación

Guzikowski NJ; Department of Pharmacology and Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA. Electronic address: natalie.j.guzikowski@vanderbilt.edu.
Kavalali ET; Department of Pharmacology and Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA. Electronic address: ege.kavalali@vanderbilt.edu.

STAR Protoc ; 4(1): 102080, 2023 03 17.

Article en En | MEDLINE | ID: mdl-36853692

RESUMEN

Visualizing the nano-organization of the synapse is fundamental to elucidating the structure-function relationship of the nervous system. The advent of super-resolution microscopy provides a tool to assess and quantify the dynamic organization of numerous proteins at the synapse. Here we present a protocol assessing inhibitory synapse scaffold protein, gephyrin, in rat primary hippocampal cultures using dSTORM microscopy. We delineate the steps for artemisinin treatment, immunocytochemistry, dSTORM image acquisition, single-molecule localization, and the analysis of synaptic scaffold protein dynamics. For complete details on the use and execution of this protocol, please refer to Guzikowski and Kavalali (2022).1.

Asunto(s)

Neuronas; Sinapsis; Ratas; Animales; Neuronas/metabolismo; Sinapsis/metabolismo; Microscopía/métodos; Hipocampo/metabolismo

Palabras clave

Microscopy; Neuroscience

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sinapsis / Neuronas Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: STAR Protoc Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google