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Bone Sialoprotein Immobilized in Collagen Type I Enhances Angiogenesis In Vitro and In Ovo.
Kriegel, Anja; Langendorf, Eva; Kottmann, Valentina; Kämmerer, Peer W; Armbruster, Franz Paul; Wiesmann-Imilowski, Nadine; Baranowski, Andreas; Gercek, Erol; Drees, Philipp; Rommens, Pol Maria; Ritz, Ulrike.
Afiliación
  • Kriegel A; Department of Orthopedics and Traumatology, BiomaTiCS, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany.
  • Langendorf E; Department of Orthopedics and Traumatology, BiomaTiCS, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany.
  • Kottmann V; Department of Orthopedics and Traumatology, BiomaTiCS, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany.
  • Kämmerer PW; Department of Oral- and Maxillofacial Surgery, BiomaTiCS, University Medical Center Mainz, 55131 Mainz, Germany.
  • Armbruster FP; Immundiagnostik AG, 64673 Bensheim, Germany.
  • Wiesmann-Imilowski N; Department of Oral- and Maxillofacial Surgery, BiomaTiCS, University Medical Center Mainz, 55131 Mainz, Germany.
  • Baranowski A; Department of Otorhinolaryngology, BiomaTiCS, University Medical Center Mainz, 55131 Mainz, Germany.
  • Gercek E; Department of Orthopedics and Traumatology, BiomaTiCS, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany.
  • Drees P; Department of Orthopedics and Traumatology, BiomaTiCS, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany.
  • Rommens PM; Department of Orthopedics and Traumatology, BiomaTiCS, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany.
  • Ritz U; Department of Orthopedics and Traumatology, BiomaTiCS, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany.
Polymers (Basel) ; 15(4)2023 Feb 17.
Article en En | MEDLINE | ID: mdl-36850289
Bone fracture healing is a multistep process, including early immunological reactions, osteogenesis, and as a key factor, angiogenesis. Molecules inducing osteogenesis as well as angiogenesis are rare, but hold promise to be employed in bone tissue engineering. It has been demonstrated that the bone sialoprotein (BSP) can induce bone formation when immobilized in collagen type I, but its effect on angiogenesis still has to be characterized in detail. Therefore, the aim of this study was to analyse the effects of BSP immobilized in a collagen type I gel on angiogenesis. First, in vitro analyses with endothelial cells (HUVECs) were performed detecting enhancing effects of BSP on proliferation and gene expression of endothelial markers. A spheroid model was employed confirming these results. Finally, the inducing impact of BSP-collagen on vascular density was proved in a yolk sac membrane assay. Our results demonstrate that BSP is capable of inducing angiogenesis and confirm that collagen type I is the optimal carrier for this protein. Taking into account former results, and literature showing that BSP also induces osteogenesis, one can hypothesize that BSP couples angiogenesis and osteogenesis, making it a promising molecule to be used in bone tissue regeneration.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Polymers (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Polymers (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza