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Tergitol Based Decellularization Protocol Improves the Prerequisites for Pulmonary Xenografts: Characterization and Biocompatibility Assessment.
Tondato, Susanna; Moro, Arianna; Butt, Salman; Todesco, Martina; Sandrin, Deborah; Borile, Giulia; Marchesan, Massimo; Fabozzo, Assunta; Bagno, Andrea; Romanato, Filippo; Imran, Saima Jalil; Gerosa, Gino.
Afiliación
  • Tondato S; Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padua, 35128 Padua, Italy.
  • Moro A; L.I.F.E.L.A.B Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, 35127 Padua, Italy.
  • Butt S; L.I.F.E.L.A.B Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, 35127 Padua, Italy.
  • Todesco M; Department of Industrial Engineering, University of Padua, 35131 Padua, Italy.
  • Sandrin D; Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padua, 35128 Padua, Italy.
  • Borile G; L.I.F.E.L.A.B Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, 35127 Padua, Italy.
  • Marchesan M; L.I.F.E.L.A.B Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, 35127 Padua, Italy.
  • Fabozzo A; Department of Industrial Engineering, University of Padua, 35131 Padua, Italy.
  • Bagno A; L.I.F.E.L.A.B Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, 35127 Padua, Italy.
  • Romanato F; Department of Physics and Astronomy "G. Galilei'', University of Padua, 35131 Padua, Italy.
  • Imran SJ; L.I.F.E.L.A.B Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, 35127 Padua, Italy.
  • Gerosa G; Department of Physics and Astronomy "G. Galilei'', University of Padua, 35131 Padua, Italy.
Polymers (Basel) ; 15(4)2023 Feb 06.
Article en En | MEDLINE | ID: mdl-36850103
Right ventricle outflow tract obstruction (RVOTO) is a congenital pathological condition that contributes to about 15% of congenital heart diseases. In most cases, the replacement of the right ventricle outflow in pediatric age requires subsequent pulmonary valve replacement in adulthood. The aim of this study was to investigate the extracellular matrix scaffold obtained by decellularization of the porcine pulmonary valve using a new detergent (Tergitol) instead of Triton X-100. The decellularized scaffold was evaluated for the integrity of its extracellular matrix (ECM) structure by testing for its biochemical and mechanical properties, and the cytotoxicity/cytocompatibility of decellularized tissue was assessed using bone marrow-derived mesenchymal stem cells. We concluded that Tergitol could remove the nuclear material efficiently while preserving the structural proteins of the matrix, but without an efficient removal of the alpha-gal antigenic epitope. Therefore, Tergitol can be used as an alternative detergent to replace the Triton X-100.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Polymers (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Polymers (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza