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The Berlin-Buch respiration chamber for energy expenditure measurements.
Mähler, Anja; Schütte, Till; Steiniger, Jochen; Boschmann, Michael.
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  • Mähler A; Experimental and Clinical Research Center, a cooperation between the Max Delbrück Center in the Helmholtz Association and Charité - Universitätsmedizin Berlin, Lindenberger Weg 80, 13125, Berlin, Germany. anja.maehler@charite.de.
  • Schütte T; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Lindenberger Weg 80, 13125, Berlin, Germany. anja.maehler@charite.de.
  • Steiniger J; Max Delbrück Center in the Helmholtz Association (MDC), Berlin, Germany. anja.maehler@charite.de.
  • Boschmann M; Experimental and Clinical Research Center, a cooperation between the Max Delbrück Center in the Helmholtz Association and Charité - Universitätsmedizin Berlin, Lindenberger Weg 80, 13125, Berlin, Germany.
Eur J Appl Physiol ; 123(6): 1359-1368, 2023 Jun.
Article en En | MEDLINE | ID: mdl-36849666
PURPOSE: We present a methodological overview of a respiration chamber at the Experimental and Clinical Research Center in Berlin, Germany. Since 2010, we investigated 750 healthy subjects and patients with various diseases. We routinely measure resting energy expenditure (REE), dietary-induced thermogenesis, and activity energy expenditure. METHODS: The chamber is a pull calorimeter with a total volume of 11,000 L. The majority of measurements is done with a flow rate of 120 L/min, yielding a favorable time constant of 1.53 h. The gas analysis system consists of two paramagnetic O2 sensors and two infrared CO2 sensors, one for incoming and one for outgoing air samples. O2 and CO2 sensors are calibrated simultaneously before each measurement with a 6 min calibration routine. To verify the accuracy of the whole the calorimetric system, it is validated every 2 weeks by 2 h acetone burning tests. RESULTS: Validation factors (calculated/measured) of 20 representative 2 h acetone burning tests were 1.03 ± 0.03 for [Formula: see text], 1.02 ± 0.02 for [Formula: see text], 0.99 ± 0.02 for RER, and 1.03 ± 0.03 for EE. Four repeated 60 min REE measurements of a healthy woman showed variabilities of 231.9 ± 4.8 ml/min for [Formula: see text] (CV 2.1%), 166.0 ± 6.3 ml/min for [Formula: see text] (CV 3.8%), 0.73 ± 0.03 for RER (CV 4.6%), and 4.55 ± 0.07 kJ/min for EE (CV 1.6%). CONCLUSIONS: The data presented show that our respiration chamber produces precise and valid EE measurements with an exceptionally fast responsiveness.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acetona / Dióxido de Carbono Tipo de estudio: Health_economic_evaluation / Observational_studies Límite: Female / Humans País/Región como asunto: Europa Idioma: En Revista: Eur J Appl Physiol Asunto de la revista: FISIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acetona / Dióxido de Carbono Tipo de estudio: Health_economic_evaluation / Observational_studies Límite: Female / Humans País/Región como asunto: Europa Idioma: En Revista: Eur J Appl Physiol Asunto de la revista: FISIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania