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Structural and biochemical insight into a modular ß-1,4-galactan synthase in plants.
Prabhakar, Pradeep Kumar; Pereira, Jose Henrique; Taujale, Rahil; Shao, Wanchen; Bharadwaj, Vivek S; Chapla, Digantkumar; Yang, Jeong-Yeh; Bomble, Yannick J; Moremen, Kelley W; Kannan, Natarajan; Hammel, Michal; Adams, Paul D; Scheller, Henrik V; Urbanowicz, Breeanna R.
Afiliación
  • Prabhakar PK; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
  • Pereira JH; Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
  • Taujale R; Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oakridge, TN, USA.
  • Shao W; Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Bharadwaj VS; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Chapla D; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
  • Yang JY; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Bomble YJ; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Moremen KW; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Kannan N; Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
  • Hammel M; Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
  • Adams PD; Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
  • Scheller HV; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
  • Urbanowicz BR; Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
Nat Plants ; 9(3): 486-500, 2023 03.
Article en En | MEDLINE | ID: mdl-36849618
Rhamnogalacturonan I (RGI) is a structurally complex pectic polysaccharide with a backbone of alternating rhamnose and galacturonic acid residues substituted with arabinan and galactan side chains. Galactan synthase 1 (GalS1) transfers galactose and arabinose to either extend or cap the ß-1,4-galactan side chains of RGI, respectively. Here we report the structure of GalS1 from Populus trichocarpa, showing a modular protein consisting of an N-terminal domain that represents the founding member of a new family of carbohydrate-binding module, CBM95, and a C-terminal glycosyltransferase family 92 (GT92) catalytic domain that adopts a GT-A fold. GalS1 exists as a dimer in vitro, with stem domains interacting across the chains in a 'handshake' orientation that is essential for maintaining stability and activity. In addition to understanding the enzymatic mechanism of GalS1, we gained insight into the donor and acceptor substrate binding sites using deep evolutionary analysis, molecular simulations and biochemical studies. Combining all the results, a mechanism for GalS1 catalysis and a new model for pectic galactan side-chain addition are proposed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicosiltransferasas / Galactanos Idioma: En Revista: Nat Plants Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicosiltransferasas / Galactanos Idioma: En Revista: Nat Plants Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido