A "T.E.S.T." hydrogel bioadhesive assisted by corneal cross-linking for in situ sutureless corneal repair.

Li, Meiyan; Wei, Ruoyan; Liu, Chang; Fang, Haowei; Yang, Weiming; Wang, Yunzhe; Xian, Yiyong; Zhang, Kunxi; He, Yong; Zhou, Xingtao

Li, Meiyan; Wei, Ruoyan; Liu, Chang; Fang, Haowei; Yang, Weiming; Wang, Yunzhe; Xian, Yiyong; Zhang, Kunxi; He, Yong; Zhou, Xingtao.

Afiliación

Li M; Department of Ophthalmology, EYE & ENT Hospital of Fudan University Shanghai, China.
Wei R; NHC Key Laboratory of Myopia (Fudan University), Shanghai, China.
Liu C; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China.
Fang H; Department of Ophthalmology, EYE & ENT Hospital of Fudan University Shanghai, China.
Yang W; NHC Key Laboratory of Myopia (Fudan University), Shanghai, China.
Wang Y; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China.
Xian Y; Department of Ophthalmology, EYE & ENT Hospital of Fudan University Shanghai, China.
Zhang K; NHC Key Laboratory of Myopia (Fudan University), Shanghai, China.
He Y; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China.
Zhou X; Department of Polymer Materials, School of Materials Science and Engineering, Shanghai University, Shanghai, China.

Bioact Mater ; 25: 333-346, 2023 Jul.

Article en En | MEDLINE | ID: mdl-36844364

RESUMEN

Corneal transplantation is an effective clinical treatment for corneal diseases, which, however, is limited by donor corneas. It is of great clinical value to develop bioadhesive corneal patches with functions of "Transparency" and "Epithelium & Stroma generation", as well as "Suturelessness" and "Toughness". To simultaneously meet the "T.E.S.T." requirements, a light-curable hydrogel is designed based on methacryloylated gelatin (GelMA), Pluronic F127 diacrylate (F127DA) & Aldehyded Pluronic F127 (AF127) co-assembled bi-functional micelles and collagen type I (COL I), combined with clinically applied corneal cross-linking (CXL) technology for repairing damaged cornea. The patch formed after 5 min of ultraviolet irradiation possesses transparent, highly tough, and strongly bio-adhesive performance. Multiple cross-linking makes the patch withstand deformation near 600% and exhibit a burst pressure larger than 400 mmHg, significantly higher than normal intraocular pressure (10-21 mmHg). Besides, the slower degradation than GelMA-F127DA&AF127 hydrogel without COL I makes hydrogel patch stable on stromal beds in vivo, supporting the regrowth of corneal epithelium and stroma. The hydrogel patch can replace deep corneal stromal defects and well bio-integrate into the corneal tissue in rabbit models within 4 weeks, showing great potential in surgeries for keratoconus and other corneal diseases by combining with CXL.

Palabras clave

AF127, Aldehyded Pluronic F127; AS-OCT, Anterior Segment Optical Coherence Tomography; Bioadhesives; CCK-8, Cell Counting Kit-8; COL I, Collagen Type I; CXL; CXL, Corneal Cross-linking; Corneal patch; DLS, Dynamic Light Scattering; DMEM, Dulbecco's Modified Eagle's Medium; ECM, Extracellular Matrix; F127DA, Pluronic F127 diacrylate; FBS, Fetal Bovine Serum; GelMA, Methacryloylated Gelatin; H&E, Hematoxylin and Eosin; IHC, Immunohistochemistry; IOP, Intraocular Pressure; PBS, Phosphate-buffered Saline; RF, Riboï¬avin-5-phosphate; ROS, Reactive Oxygen Species; SD, Standard Deviation; Sutureless repair; TEM, Transmission Electron Microscopy; Tough hydrogel; UV, Ultraviolet; α-SMA, Alpha Smooth Muscle Actin

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bioact Mater Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: China

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