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Comparative transcriptome and metabolome analyses of cherry leaves spot disease caused by Alternaria alternata.
Pan, Liu-Yi; Zhou, Jing; Sun, Yan; Qiao, Bai-Xue; Wan, Tian; Guo, Rui-Quan; Zhang, Juan; Shan, Dong-Qian; Cai, Yu-Liang.
Afiliación
  • Pan LY; College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
  • Zhou J; College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
  • Sun Y; College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
  • Qiao BX; College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
  • Wan T; College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
  • Guo RQ; College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
  • Zhang J; College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
  • Shan DQ; College of Horticulture and Forestry, Tarim University, Alar, Xinjiang, China.
  • Cai YL; College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
Front Plant Sci ; 14: 1129515, 2023.
Article en En | MEDLINE | ID: mdl-36844070
Alternaria alternata is a necrotrophic fungal pathogen with a broad host range that causes widespread and devastating disease in sweet cherry (Prunus avium). We selected a resistant cultivar (RC) and a susceptible cultivar (SC) of cherry and used a combined physiological, transcriptomic, and metabolomic approach to investigate the molecular mechanisms underlying the plant's resistance to A. alternata, of which little is known. We found that A. alternata infection stimulated the outbreak of reactive oxygen species (ROS) in cherry. The responses of the antioxidant enzymes and chitinase to disease were observed earlier in the RC than in the SC. Moreover, cell wall defense ability was stronger in the RC. Differential genes and metabolites involved in defense responses and secondary metabolism were primarily enriched in the biosynthesis of phenylpropanoids, tropane, piperidine and pyridine alkaloids, flavonoids, amino acids, and α-linolenic acid. Reprogramming the phenylpropanoid pathway and the α-linolenic acid metabolic pathway led to lignin accumulation and early induction of jasmonic acid signaling, respectively, in the RC, which consequently enhanced antifungal and ROS scavenging activity. The RC contained a high level of coumarin, and in vitro tests showed that coumarin significantly inhibited A. alternata growth and development and had antifungal effect on cherry leaves. In addition, differentially expressed genes encoding transcription factors from the MYB, NAC, WRKY, ERF, and bHLH families were highly expressed, they could be the key responsive factor in the response of cherry to infection by A. alternata. Overall, this study provides molecular clues and a multifaceted understanding of the specific response of cherry to A. alternata.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Plant Sci Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Plant Sci Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza