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Detection of anti-feline infectious peritonitis virus activity of a Chinese herb extract using geneLEAD VIII, a fully automated nucleic acid extraction/quantitative PCR testing system.
Nishijima, Rui; Endo, Takuro; Gankhuyag, Enkhjavkhlan; Khin, Shwe Thiri Maung Maung; Jafar, Sheikhy Mohammad; Shinohara, Yuta; Tanaka, Yoshikazu; Sawakami, Kazumi; Yohda, Masafumi; Furuya, Tetsuya.
Afiliación
  • Nishijima R; Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
  • Endo T; Laboratory of Veterinary Infectious Diseases, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
  • Gankhuyag E; Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
  • Khin STMM; Laboratory of Veterinary Infectious Diseases, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
  • Jafar SM; Laboratory of Veterinary Infectious Diseases, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
  • Shinohara Y; Iskra Industry Co., Ltd., Tokyo, Japan.
  • Tanaka Y; Department of Veterinary Hygiene, Veterinary School, Nippon Veterinary and Life Science University, Tokyo, Japan.
  • Sawakami K; Precision System Science Co., Ltd., Chiba, Japan.
  • Yohda M; Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
  • Furuya T; Laboratory of Veterinary Infectious Diseases, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
J Vet Med Sci ; 85(4): 443-446, 2023 Mar 30.
Article en En | MEDLINE | ID: mdl-36806247
The geneLEAD VIII is a fully-automated nucleic acid extraction/quantitative PCR equipment developed by Precision System Science Co., Ltd., (PSS). To take advantage of its capability, we developed a quantitative assay system to measure growth of animal viruses. The system was used to assay one of the Chinese herbal extracts whose anti-malarial activities were previously reported and demonstrated its dose-dependent anti-viral activity against feline infectious peritonitis virus (FIPV), a feline coronavirus causing the fatal diseases in cats, and relatively low cell toxicity. The assay developed in this study is useful to screen antiviral drugs and the anti-FIPV activity of the herbal extract identified have a potential to lead to development of new drugs against FIPV and other coronaviruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Peritonitis / Enfermedades de los Gatos / Coronavirus Felino / COVID-19 / Antineoplásicos Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Vet Med Sci Asunto de la revista: MEDICINA VETERINARIA Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Peritonitis / Enfermedades de los Gatos / Coronavirus Felino / COVID-19 / Antineoplásicos Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Vet Med Sci Asunto de la revista: MEDICINA VETERINARIA Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón