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Toxic and antigenic characterization of Peruvian Micrurus surinamensis coral snake venom.
Oliveira, Daysiane de; Guerra-Duarte, Clara; Stransky, Stephanie; Scussel, Rahisa; Pereira de Castro, Karen Larissa; Costal-Oliveira, Fernanda; Aragão, Matheus; Oliveira-Souza, Gladstony de; Saavedra-Langer, Rafael; Trevisan, Gabriela; Bonilla-Ferreyra, Cesar; Chávez-Olórtegui, Carlos; Machado-de-Ávila, Ricardo Andrez.
Afiliación
  • Oliveira D; Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, Brazil.
  • Guerra-Duarte C; Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, Brazil.
  • Stransky S; Department of Biochemistry, Albert Einstein College of Medicine, New York, United States.
  • Scussel R; Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, Brazil.
  • Pereira de Castro KL; Departament of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, United States.
  • Costal-Oliveira F; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Aragão M; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Oliveira-Souza G; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Saavedra-Langer R; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Trevisan G; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil.
  • Bonilla-Ferreyra C; Instituto Nacional de Salud, Lima, Peru.
  • Chávez-Olórtegui C; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address: olortegi@icb.ufmg.br.
  • Machado-de-Ávila RA; Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, Brazil. Electronic address: r_andrez@unesc.net.
Toxicon ; 225: 107056, 2023 Mar 15.
Article en En | MEDLINE | ID: mdl-36804442
Micrurus surinamensis is a semi-aquatic coral snake found in primary forest region and can cause relevant human accidents. In this work we investigated the toxic and antigenic activities of the Peruvian Micrurus surinamensis venom (MsV). We found that MsV show hyaluronidase activity but lack LAAO and PLA2 enzymatic activities. Interestingly, MsV induce edematogenic responses but cannot cause nociceptive effects. Furthermore, MsV can reduce in vitro cell viability in MGSO-3 cell line derived from human breast cancer tissue. To evaluate its antigenic potential, rabbits were immunized with MsV, which proved to be immunogenic. ELISA, immunobloting and in vivo neutralization assays demonstrated that the specific rabbit anti-MsV antivenom is more efficient than the therapeutic Brazilian antivenom in recognizing and neutralizing the lethal activity of MsV. MsV differs in protein profile and biological activities from M. frontalis venom (MfV), used as control, which impairs its recognition and neutralization by Brazilian therapeutic anti-elapidic antivenom. We performed a SPOT immunoassay for the identification of B-cell linear epitopes in the main toxins described for MsV targeted by the elicited neutralizing antibodies previously produced. A membrane containing 15-mer peptides representing the sequences of five 3TFxs and five PLA2s was produced and probed with anti- MsV antibodies. Results revealed important regions in 3FTx toxins for venom neutralization. Identifying the main MsV components and its biological activities can be helpful in guiding the production of antivenoms and in the optimization of treatment for coral snake envenomation in Brazil.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxinas Biológicas / Serpientes de Coral Límite: Animals / Humans País/Región como asunto: America do sul / Caribe ingles / Peru / Suriname Idioma: En Revista: Toxicon Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxinas Biológicas / Serpientes de Coral Límite: Animals / Humans País/Región como asunto: America do sul / Caribe ingles / Peru / Suriname Idioma: En Revista: Toxicon Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido