An amplification-free CRISPR/Cas12a-based fluorescence assay for ultrasensitive detection of nuclease activity.

Wang, Xinping; Chen, Yichuan; Ma, Lixin; Han, Zhenwei; Liu, Yi; Qiao, Jie

Wang, Xinping; Chen, Yichuan; Ma, Lixin; Han, Zhenwei; Liu, Yi; Qiao, Jie.

Afiliación

Wang X; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, China.
Chen Y; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, China.
Ma L; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, China.
Han Z; BravoVax Co., Ltd., Wuhan, Hubei, China.
Liu Y; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, China; State Key Laboratory of Esophageal Cancer Prevention and Treatment, Zhengzhou University, Zhengzhou, Henan, China; BravoVax Co., Ltd., Wuhan, Hubei, China. Electronic address:
Qiao J; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, China; School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei, China; State Key Laboratory of Esophageal Cancer Prevention and Treatment, Zhengzhou Univers

Talanta ; 257: 124329, 2023 May 15.

Article en En | MEDLINE | ID: mdl-36801553

Asunto(s)

Bioensayo; Sistemas CRISPR-Cas; Humanos; ADN de Cadena Simple; Desoxirribonucleasa I; Endonucleasas; ARN Guía de Sistemas CRISPR-Cas; Ribonucleasa H

Palabras clave

Amplification-free; CRISPR/Cas12a; DNase I; Fluorescence assay; RNase H

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bioensayo / Sistemas CRISPR-Cas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Talanta Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos

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