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Initial spindle positioning at the oocyte center protects against incorrect kinetochore-microtubule attachment and aneuploidy in mice.
Kincade, Jessica N; Hlavacek, Avery; Akera, Takashi; Balboula, Ahmed Z.
Afiliación
  • Kincade JN; Animal Sciences Research Center, University of Missouri, Columbia, MO 65211, USA.
  • Hlavacek A; Animal Sciences Research Center, University of Missouri, Columbia, MO 65211, USA.
  • Akera T; Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
  • Balboula AZ; Animal Sciences Research Center, University of Missouri, Columbia, MO 65211, USA.
Sci Adv ; 9(7): eadd7397, 2023 02 17.
Article en En | MEDLINE | ID: mdl-36800430
Spindle positioning within the oocyte must be tightly regulated. In mice, the spindle is predominantly assembled at the oocyte center before its migration toward the cortex to achieve the highly asymmetric division, a characteristic of female meiosis. The significance of the initial central positioning of the spindle is largely unknown. We show that initial spindle positioning at the oocyte center is an insurance mechanism to avoid the premature exposure of the spindle to cortical CDC42 signaling, which perturbs proper kinetochore-microtubule attachments, leading to the formation of aneuploid gametes. These findings contribute to understanding why female gametes are notoriously associated with high rates of aneuploidy, the leading genetic cause of miscarriage and congenital abnormalities.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cinetocoros / Huso Acromático Límite: Animals Idioma: En Revista: Sci Adv Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cinetocoros / Huso Acromático Límite: Animals Idioma: En Revista: Sci Adv Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos