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Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas.
Sprankel, Lasse; Vizarraga, David; Martín, Jesús; Manger, Sina; Meier-Credo, Jakob; Marcos, Marina; Julve, Josep; Rotllan, Noemi; Scheffer, Margot P; Escolà-Gil, Joan Carles; Langer, Julian D; Piñol, Jaume; Fita, Ignacio; Frangakis, Achilleas S.
Afiliación
  • Sprankel L; Buchmann Institute for Molecular Life Sciences and Institute of Biophysics, Goethe University Frankfurt, Frankfurt, Germany.
  • Vizarraga D; Instituto de Biología Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Spain.
  • Martín J; Instituto de Biología Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Spain.
  • Manger S; Buchmann Institute for Molecular Life Sciences and Institute of Biophysics, Goethe University Frankfurt, Frankfurt, Germany.
  • Meier-Credo J; Proteomics, Max Planck Institute of Biophysics, Frankfurt, Germany.
  • Marcos M; Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.
  • Julve J; Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau and CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Barcelona, Spain.
  • Rotllan N; Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau and CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Barcelona, Spain.
  • Scheffer MP; Buchmann Institute for Molecular Life Sciences and Institute of Biophysics, Goethe University Frankfurt, Frankfurt, Germany.
  • Escolà-Gil JC; Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau and CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Barcelona, Spain.
  • Langer JD; Proteomics, Max Planck Institute of Biophysics, Frankfurt, Germany.
  • Piñol J; Proteomics, Max Planck Institute for Brain Research, Frankfurt, Germany.
  • Fita I; Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.
  • Frangakis AS; Instituto de Biología Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Barcelona, Spain. ifrcri@ibmb.csic.es.
Nat Struct Mol Biol ; 30(3): 321-329, 2023 03.
Article en En | MEDLINE | ID: mdl-36782049
Mycoplasma pneumoniae, responsible for approximately 30% of community-acquired human pneumonia, needs to extract lipids from the host environment for survival and proliferation. Here, we report a comprehensive structural and functional analysis of the previously uncharacterized protein P116 (MPN_213). Single-particle cryo-electron microscopy of P116 reveals a homodimer presenting a previously unseen fold, forming a huge hydrophobic cavity, which is fully accessible to solvent. Lipidomics analysis shows that P116 specifically extracts lipids such as phosphatidylcholine, sphingomyelin and cholesterol. Structures of different conformational states reveal the mechanism by which lipids are extracted. This finding immediately suggests a way to control Mycoplasma infection by interfering with lipid uptake.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adhesinas Bacterianas / Mycoplasma pneumoniae Límite: Humans Idioma: En Revista: Nat Struct Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adhesinas Bacterianas / Mycoplasma pneumoniae Límite: Humans Idioma: En Revista: Nat Struct Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos