Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq) Technology.
Methods Mol Biol
; 2638: 37-57, 2023.
Article
en En
| MEDLINE
| ID: mdl-36781634
Double digest restriction-site associated DNA sequencing (ddRADseq) technology combines genome reduced representation by digestion with two restriction enzymes and next generation sequencing (NGS) to obtain thousands of markers (SNP, SSR, and InDels) and genotype tens to hundreds of samples simultaneously. In this chapter, we describe a 96-plex derived ddRADseq protocol that can be set up to obtain different depth of coverage per locus and can be exploited to model and non-model plant species.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genoma
/
Secuenciación de Nucleótidos de Alto Rendimiento
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2023
Tipo del documento:
Article
País de afiliación:
Argentina
Pais de publicación:
Estados Unidos